Skip to main content

Culture of Animal Cells: A Manual of Basic Technique, 5th Edition

Culture of Animal Cells: A Manual of Basic Technique, 5th Edition

R. Ian Freshney

ISBN: 978-0-471-74759-8

Oct 2005

696 pages

Select type: O-Book

Description

The most complete resource on the techniques, equipment,principles, and practices of animal cell culture

Since publication of the previous edition of this benchmark text, numerous groundbreaking advances have occurred in stem cell research, cloning, tissue engineering, and in vitro toxicity testing. These and other developments have been incorporated into this fully revised and expanded Fifth Edition of Culture of Animal Cells. In addition, to answer the needs of the exponential increase in newcomers to cell culture, particularly in the biopharmaceutical industry, a completely new chapter on training in cell culture technology has been introduced.

The most complete resource on the techniques, equipment, principles, and practices of animal cell culture, this text offers a complete background related to growth of animal cells in culture. Beginning with laboratory design, safety, validation and bioethics, then continuing with preparation of media, primary culture and cell lines, through to characterization and authentication, contamination, specialized techniques, and troubleshooting, the coverage includes:
* An all-new section of training exercises, separated into basic, intermediate, and advanced procedures, cross-referenced to the relevant protocols
* New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro toxicity testing, and tissue engineering
* An expanded full-color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, contamination, and specialized equipment
* Enhanced treatment of troubleshooting, with full cross-referencing to the relevant protocols and sections of text
* Fully updated references
* The clearest, most consistent presentation of step-by-step protocols available
* Numerous diagrams, photographs, tables, and charts
* Detailed and up-to-date information on reagent preparation and sourcing of materials and equipment, including a fully updated list of suppliers and other resources with Web sites

Indispensable for clinical and biopharmaceutical researchers and scientists, students, trainees, and technicians, this landmark text presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells.
List of Figures.

List of Color Plates.

Preface.

Abbreviations.

1. Introduction.

2. Training Programs.

3. Biology of Cultured Cells.

4. Laboratory Design and Layout.

5. Equipment.

6. Aseptic Technique.

7. Safety, Bioethics, and Validation.

8. Culture Vessels and Substrates.

9. Defined Media and Supplements.

10. Serum-Free Media.

11. Preparation and Sterilization.

12. Primary Culture.

13. Subculture and Cell Lines.

14. Cloning and Selection.

15. Cell Separation.

16. Characterization.

17. Differentiation.

18. Transformation and Immortalization.

19. Contamination.

20. Cryopreservation.

21. Quantitation.

22. Cytotoxocity.

23. Culture of Specific Cell Types.

24. Culture of Tumor Cells.

25. Organotypic Culture.

26. Scale-Up.

27. Specialized Techniques.

28. Problem Solving.

29. In Conclusion.

Appendix I: Preparation of Reagents.

Appendix II: Sources of Equipment and Materials.

Appendix III: Suppliers and Other Resources.

Appendix IV: Glossary.

Appendix V: General Textbooks and Relevant Journals.

References.

Index.

""...a good user friendly resource...a handy reference book for routing cell culture protocols.""  (In Vitro Cellular & Development Biology - Animal, May/June 2006)
  • All new section of training exercises, separated into basic, intermediate, and advanced procedures
  • New coverage of stem cells, bioethics, validation, cloning, cell signaling, in vitro cosmetic testing, and tissue engineering
  • Expanded full color atlas section, with images of primary culture, cell lines, subculture, differentiation, cancer cells and transformation, three-dimensional culture, and contamination
  • Enhanced treatment of trouble-shooting, with new discussion of frequency of subculture, cell concentration, and photosensitivity
  • Now includes a list of related websites
  • Fully updated references
  • Provides the clearest, most consistent presentation of step-by-step protocols available
  • Richly illustrated with diagrams, photographs, tables, and charts
  • Includes expert insights regarding safety, ethics, and intellectual property
  • Offer detailed and up-to-date information on reagent preparation and equipment, including vendor lists