List of Abbreviations.
PART I: CELL CULTURE.
1. Basic Principles of Cell Culture (R. Freshney).
2. Mesenchymal Stem Cells for Tissue Engineering (D. Lennon & A. Caplan).
3. Human Embryonic Stem cell Culture for Tissue Engineering (S. Levenberg, et al.).
4. Cell Sources for Cartilage Tissue Engineering (B. Johnstone, et al.).
5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering (H. Madry).
PART II: TISSUE ENGINEERING.
6. Tissue Engineering: Basic Considerations (G. Vunjak-Novakovic).
7. Tissue Engineering of Articular Cartilage (K. Masuda & R. Sah).
8. Ligament Tissue Engineering (J. Chen, et al.).
9. Cellular Photoencapsulation in Hydrogels (J. Elisseeff, et al.).
10. Tissue Engineering Human Skeletal Muscle for Clinical Applications (J. Shansky, et al.).
11. Engineered Heart Tissue (T. Eschenhagen & W. Zimmermann).
12. Tissue-Engineered Blood Vessels (R. Klinger & L. Niklason).
13. Tissue Engineering of Bone (S. Hofmann, et al.).
14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering (P. Lelkes, et al.).
15. Tissue Engineering of the Liver (G. Underhill, et al.).
"The editors have brought together an outstanding group of experts to describe cell culture methods and applications for tissue engineering." (Doody's Health Services)
- Easy to use format with a two-part organization.
- Logically organized, part one discussing cell sourcing, preparation and characterization and the second part looking at specific engineered tissues.
- Integrates theoretical background and detailed step-by-step protocols for each system or application.
- Each chapter covers: structural and functional properties of tissues, methodological principles, culture, cell selection/expansion, cell modifications, cell seeding, tissue culture, analytical assays, and a detailed description of representative studies
- End-of-chapter features include useful listings of sources for reagents, materials, and supplies