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Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution

Evolutionary Methods in Biotechnology: Clever Tricks for Directed Evolution

Susanne Brakmann (Editor), Andreas Schwienhorst (Editor)

ISBN: 978-3-527-30799-9

Jul 2004

227 pages

Out of stock

$273.00

Description

Miniturization and high throughput assay technology have brought the power of molecular evolution to the bioscience laboratory. Applied wisely, the evolutionary approach can quickly yield the desired result even where other methods have failed.
From library generation by random or directed mutagenesis to screening and selection techniques -- the crucial steps for successful evolutionary biotechnology are described in detail in this practical guide that also includes valuable troubleshooting hints on frequently encountered problems.
Modern methods for the surface display of peptides and proteins, selective enrichment of nucleic acid aptamers and high-throughput screening of industrial biocatalysts are explained, and computer-based methods for in silico protein and RNA engineering are described as an alternative to in vitro approaches. A special section covers the patenting regulations with regard to biotechnological innovations derived from directed evolution.
As an added bonus, a CD-ROM is included that contains software tools for library design, selection of mutagenesis positions, and various predictive algorithms.
In short, this practice oriented handbook is an indispensable tool for every scientist working in this interdisciplinary research area.

Introduction
Generation of Mutant Libraries Using Random Mutagenesis
DNA Shuffling
DNA Recombination Using StEP
FACS Screening of Combinatorial Peptide and Protein Libraries
Selection of Phage-displayed Enzymes
Selection of Aptamers
Methods for Selecting Catalytic Nucleic Acids
High-Throughput Screening of Enantioselective Industrial Biocatalysts
Computer-assisted Design of Doped Libraries
Directed in silico Mutagenesis
RNA Folding in silico
Patenting in Evolutionary Biotechnology
Programs on CD: How to identify positions suited for mutagenesis;
Folding aspects; databases; structure-based design; and more
Glossary and Index
"From library generation by random or directed mutagenesis to screening and selection techniques, the crucial steps for succesful evolutionary biotechnology are described in detail in this practical guide, that also includes valuable troubleshooting hints on frequently encountered problems." Process

"... contains valuable information." Molecular Biotechnology

"... is truly a highly practical handbook, a "must have" for any laboratory practicing directed evolution of proteins, and a methodological "cookbook" of high quality." Angewandte Chemie I.E.

"...this volume can be recommended to novices desirous to get started with evolutionary methods as well as experienced scientists of the field." Carbohydrate Polymers

"... a useful snapshot of the state of the art of directed evolution methods for academia and industrial working groups." The Quarterly Review of Biology

"... a terrific collection of useful tricks and protocols that can be wholeheartedly recommended." ASM News