DescriptionNinfa/Ballou/Benore is a solid biochemistry lab manual, dedicated to developing research skills, allowing students to learn techniques and develop the the critical thinking and organizational approaches necessary to conduct laboratory research.
Ninfa/Ballou/Benore focuses on basic biochemistry laboratory techniques but also includes molecular biology exercises, a reflection of most courses which concentrate on traditional biochemistry experiments and techniques. The experiments are designed so that theory and technique are learned as fundamental research tools, and the biochemistry and molecular biology applications are seamlessly integrated throughout the manual. The manual also includes an introduction to ethics in the laboratory, uncommon in similar manuals. Most importantly, perhaps, is the authors’ three-pronged approach to encouraging students to think like a research scientist: first, the authors introduce the scientific method and the hypothesis as a framework for developing conclusive experiments; second, the manual’s experiments are designed to become increasingly complex in order to teach more advanced techniques and analysis; finally, gradually, the students are required to devise their own protocols. In this way, students and instructors are able to break away from a “cookbook” approach and to think and investigate for themselves.
Suitable for lower-level and upper-level courses; Ninfa spans these courses and can also be used for some first-year graduate work.
Chapter 2: Basic Procedures in the Biochemistry Laboratory
Chapter 3: Spectroscopic Methods
Chapter 4: Quantification of Protein Concentration
Chapter 5: Chromatography
Chapter 6: Gel Electrophoresis of Proteins
Chapter 7: Overview of Protein Purification
Chapter 8: Subcellular Fractionation
Chapter 9: Isolation and Characterization of an Enzyme-Alkaline Phosphatase from Escherichia Coli
Chapter 10: Enzyme Kinetics
Chapter 11: Ligand Binding
Chapter 12: Enzymatic Methods of Analysis
Chapter 13: Recombinant DNA Techniques
Chapter 14: Polymerase Chain Reaction (PCR) Technology
Chapter 15: Using the Computer and the Internet for Biochemical Research and Communication
- Inclusion of new material from research and in technology
- Suitable for lower-level and upper-level courses and can also be used for some first-year graduate work
- Dedicated to developing research skills in students, allowing them to learn techniques and develop the organizational approaches necessary to conduct laboratory research
- Includes an introduction to ethics in the laboratory
- Authors’ three-pronged approach to encouraging students to think like a research scientist