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Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging

Douglas B. Murphy

ISBN: 978-0-471-23429-6

Jun 2002

384 pages

Select type: E-Book



Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image.

Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as:

  • Illuminators, filters, and isolation of specific wavelengths
  • Phase contrast and differential interference contrast
  • Properties of polarized light and polarization microscopy
  • Fluorescence and confocal laser scanning microscopy
  • Digital CCD microscopy and image processing

Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions.

An essential resource for both, experienced and novice microscopists.


Fundamentals of Light Microscopy.

Light and Color.

Illuminators, Filters, and Isolation of Specific Wavelengths.

Lenses and Geometrical Optics.

Diffraction and Interference in Image Formation.

Diffraction and Spatial Resolution.

Phase Contrast Microscopy and Dark-Field Microscopy.

Properties of Polarized Light.

Polarization Microscopy.

Differential Interference Contrast (DIC) Microscopy and Modulation Contrast Microscopy.

Fluorescence Microscopy.

Confocal Laser Scanning Microscopy.

Video Microscopy.

Digital CCD Microscopy.

Digital Image Processing.

Image Processing for Scientific Publication.

Appendix I.

Appendix II.

Appendix III.



"...will greatly advance your knowledge of practical light imaging techniques..." (IEEE Electrical Insulation Magazine, July/August 2007)

"...To conclude, it is an outstanding book!" (Fundamentals of Light Microscopy and Electronic Imaging Microscopy and Analysis, July 2002)

"Both theoretical and practical aspects of the subject are treated with economy, clarity, and authority....Murphy?s book is unusual because it provides a technically sound and simply conceived introduction to virtually every link in the chain of technologies that makes modern imaging so powerful....This book will provide individuals without background knowledge in optical physics, electronics, or image processing with many of the basic facts they need to know to understand both the power and the limitations of their images." --J. Richard McIntosh, Cell Biology Education