Skip to main content

Green Fluorescent Protein: Properties, Applications and Protocols, 2nd Edition

Green Fluorescent Protein: Properties, Applications and Protocols, 2nd Edition

Martin Chalfie (Editor), Steven R. Kain (Editor)

ISBN: 978-0-471-73948-7

Nov 2005

450 pages



Since the discovery of the gene for green fluorescent protein (GFP), derived from jellyfish, this protein that emits a green glow has initiated a revolution in molecular biosciences. With this tool, it is now possible to visualize nearly any protein of interest in any cell or tissue of any species. Since the publication of the first edition, there have been tremendously significant technological advances, including development of new mutant variants. Proteins are now available in yellow and blue, and Novel Fluorescent Proteins (NFPs) have expanded their utility in developing biosensors, biological markers, and other biological applications.

This updated, expanded new edition places emphasis on the rise of NFPs, including new chapters on NFP properties with detailed protocols, applications of GFPs and NFPs in industry research, and biosensors. This book provides a solid theoretical framework, along with detailed, practical guidance on use of GFPs and NFPs with discussion of potential pitfalls. The expert contributors provide real examples in showing how to tailor GFP/NFP to specific systems, maximize expression, and enhance detection.
First Edition Preface.



1. Discovery of Green Fluorescent Protein (Osamu Shimomura).

2. Photons for Reporting Molecular Events: Green Fluorescent Protein and Four Luciferase Systems (J. Woodland Hastings and James G. Morin).

3. Biochemical and Physical Properties opf Green Fluorescent Protein (William W. Ward).

4. The Three-Dimensional Structure of Green Fluorescent Protein and Its Implications for Function and Design (George N. Phillips).

5. Molecular Biology and Mutation of Green Fluorescent Protein (David A. Zacharias and Roger Y. Tsien).

6. Discovery and Properties of GFP-Like Proteins from Nonbioluminescent Anthozoa (Konstantin A. Lukyanov, Dmitry M. Chudakov, Arkady F. Fradkov, Yulii A. Labas, Mikhail V. Matz, and Sergey Lukyanov).

7. Evolution of Function and Color in GPF-Like Proteins (Mikhail V. Matz, Yulii A. Labas, and Juan Ugalde).

8. The Uses of Green Fluorescent Protein in Prokaryotes (Raphael H. Valdivia, Brendan P. Cormack, and Stanley Falkow).

9. The uses of Green Fluorescent Protein in Yeasts (Amy L. Hitchcock, Jason A. Kahana, and Pamela A. Silver).

10. Uses of GFP in Caenorhabditis Elegans (Oliver Hobert and Paula Loria).

11. Green Fluorescent Protein Applications in Drosophila (Tulle Hazelrigg and Jennifer H. Mansfield).

12.The Uses of Green Fluorescent Protein in Plants (Jim Haseloff and Kirby R. Siemering).

13. Uses of GFP in Transgenic Vertebrates (Sean Magason, Adam Amsterdam, Nancy Hopkins, and Shuo Lin).

14. The Uses of Green Fluorescent Protein in Mammalian Cells (Theresa H. Ward and Jennifer Lippincott-Schwartz).

15. Practical Considerations for Use of Reef Coral Fluorescent Proteins in Mammalian Cells: Applications in Fluorescent Microscopy and Flow Cytometry (Yu Fang, Olivier Déry, Michael Haugwitz, Pierre Turpin, and Steven R. Kain).

16. Pharmaceutical Applications of GFP and RCFP (Nicola Bevan and Stephen Rees).

17. Reassembled GFP: Detecting Protein-Protein Interactions and Protein Expression Patterns (Thomas J. Magliery and Lynne Regan).

Methods and Protocols (Steven R. Kain).


"...a magnificent text that will immensely benefit students and scientists from many biomedical areas." (Annals of Biomedical Engineering, August 2006)

"Any researcher, graduate student or educator with an interest in using GFP should read this book. It will become the 'bible for GFP' users." (The Quarterly Review of Biology, June 2006)