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HPLC Made to Measure: A Practical Handbook for Optimization

HPLC Made to Measure: A Practical Handbook for Optimization

Stavros Kromidas (Editor)

ISBN: 978-3-527-61199-7

Jun 2008

786 pages



The only topical HPLC book to focus on optimization, this volume addresses the needs of HPLC users who wish to constantly improve their methods, in particular in terms of throughput, accuracy and cost-effectiveness.
This handbook features contributions from such bestselling authors as John W. Dolan, Michael McBrien, Veronika R. Meyer, Uwe D. Neue, Lloyd R. Snyder, and Klaus K. Unger, as well as from scientists working for major companies, including Agilent, AstraZeneca, Merck, Schering, Tosoh Biosep, VWR, and Waters. It covers essential aspects of optimization in general, optimization in different LC-modi, hyphenated techniques and computer-aided optimization. The whole is rounded off with a section of user reports.
Principles of HPLC Optimization Illustrated by RP-Chromatography
Fast Gradient Separations
pH and Selectivity in Reversed-Phase Chromatography
Selecting the Correct pH-Value for HPLC
Optimization of Data Evaluation in Chromatography
Calibration Characteristics and Uncertainty indicating Starting-Points for Method Optimization
Comparison and Choice of Columns for RP-HPLC
Column Selectivity in Reversed-Phase Chromatography
The Use of Principal Component Analysis for the Characterisation of Reversed-Phase Liquid Chromatographic Stationary Phases
Chemometrics -
A Powerful Tool for Handling a Large Number of Data
Linear Free Energy Relations -
Tools for Column Characterization and Method Optimization in HPLC?
Column Selectivity in Reversed-Phase Liquid Chromatography
Understanding Selectivity by Use of Suspended-State High Resolution Magic Angle Spinning NMR Spectroscopy
Optimization in Normal-Phase HPLC
Optimization of GPC/SEC Separations by Proper Selection of Stationary Phase and Detection
Gel Filtration -
Size Exclusion Chromatography of biopolymers
Optimization in Affinity Chromatography
Optimization of Chiral Separations in HPLC
µLC/nano LC -
Optimization and Troubleshooting
Microchip-Based Liquid Chromatography -
Techniques and Possibilities
Ultra-Performance Liquid Chromatography
Immunochromatographic Techniques
Enhanced Characterization and Comprehensive Analyses by 2-dimensional Chromatography
Hints and Recommendations for Optimization and Troubleshooting
Computer-Facilitated HPLC Method Development Using DryLab Software
ChromSword Software for Automated and Computer-Assisted Development of HPLC Methods
Multifactorial Systematic Method Development and Optimization in Reversed Phase HPLC
NanoLC-MS/MS in Proteomics
Ways for the Verification of the Robustness in RP-HPLC
Separation of Complex Sample Mixtures
Evaluation of an integrated procedure for the characterization of chemical libraries on the basis of HPLC-UV/MS/CLND
""A valuable text by any estimation, I would recommend it highly for hands-on users or managers directing a group of chromatographers toward more efficient and effective HPLC separations."" (Journal of the American Chemical Society, February 21, 2007)