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Handbook of Chemical and Biological Plant Analytical Methods, 3 Volume Set

Handbook of Chemical and Biological Plant Analytical Methods, 3 Volume Set

Kurt Hostettmann (Editor-in-Chief), Shilin Chen (Editor), Andrew Marston (Editor), Hermann Stuppner (Editor)

ISBN: 978-1-118-87498-1

Jul 2014

1176 pages



Plants and plant-derived compounds and drugs are becoming more and more popular with increasing numbers of scientists researching plant analysis. The quality control of herbal drugs is also becoming essential to avoid severe health problems, and in the future many more new drugs will be developed from plant sources.

This three-volume Handbook, featuring 47 detailed review articles, is unique as it deals with chemical and biological methodologies for plant analysis. It presents the most important and most accurate methods which are available for plant analysis.

This comprehensive work is divided into six sections as follows:

Sample preparation and identification – discussing plant selection and collection, followed by extraction and sample preparation methodologies.

Extraction and sample preparation methodologies

Instrumentation for chemical analysis - several instrumentations for chemical plant analysis are presented with an emphasis on hyphenated techniques, e.g. the coupling between HPLC and mass spectroscometry, and HPLC with NMR.

Strategies for selective classes of compounds – coverage of the most interesting classes of compounds such as polysaccharides, saponins, cardiotonic glycosides, alkaloids, terpenoids, lipids, volatile compounds and polyphenols ( flavonoids, xanthones, coumarins, naphthoquinones, anthraquinones, proanthocyanidins, etc.).

Biological Analysis - includes phenotyping, DNA barcoding techniques, transcriptome analysis , microarray, metabolomics and proteomics.

Drugs from Plants – covers the screening of plant extracts  and strategies for the quick discovery of novel bioactive natural products. Safety assessment of herbal drugs is highly dependent on outstanding chromatographic and spectroscopic methods which are also featured here.

This Handbook introduces to scientists involved in plant studies the current knowledge of methodologies in various fields of chemically- and biochemically-related topics in plant research.

The content from this Handbook will publish online within the Encyclopedia of Analytical Chemistry via Wiley Online Library:

Benefit from the introductory offer, valid until 30 November 2014!
Introductory price: £425.00 / $695.00 / €550.00
List price thereafter: £495.00 / $795.00 / €640.00


List of Contributors xi

Preface xvii

In Memoriam
Andrew Marston xix

Part One: Sample Preparation and Identification 1

1 Selection, Identification, and Collection of Plants 3
Monique S. J. Simmonds

2 Extraction Methodologies: General Introduction 17
Valerie Camel

3 Supercritical Fluid Extraction 43
Seied Mahdi Pourmortazavi, Mehdi Rahimi-Nasrabadi and Somayeh Mirsadeghi

4 New Trends in Extraction of Natural Products: Microwave-Assisted Extraction and Pressurized Liquid Extraction 77
Philippe Christen and Beatrice Kaufmann

5 Solid-Phase Microextraction (SPME) and Its Application to Natural Products 105
M. Abdul Mottaleb, Mohammed J. Meziani and M. Rafiq Islam

Part Two: Instrumentation for Chemical Analysis 129

6 Microscopic Analysis 131
Johannes Saukel and Elisabeth Ginko

7 Thin-Layer Chromatography, with Chemical and Biological Detection Methods 185
Aurelie Urbain and Claudia Avello Simoes-Pires

8 HPLC and Ultra HPLC: Basic Concepts 207
Veronika R. Meyer

9 Near-Infrared (NIR) Spectroscopy in Natural Product Research 227
Christian W. Huck

10 Headspace Sampling and Gas Chromatography: A Successful Combination to Study the Composition of a Plant Volatile Fraction 245
Barbara Sgorbini, Cecilia Cagliero, Chiara Cordero, Erica Liberto, Patrizia Rubiolo and Carlo Bicchi

11 Analysis of Natural Products by Capillary Electrophoresis and Related Techniques 277
Markus Ganzera and Anja Kruger

12 LC and LC-MS: Techniques and Applications 307
Nadja Arens, Stefanie Doell and Hans-Peter Mock

13 NMR as Analytical Tool for Crude Plant Extracts 317
Anna R. Bilia

14 NMR of Small Molecules 349
Christoph Seger

15 NMR of Large Molecules 361
Christoph H. Wunderlich, Sarina Grutsch, Martin Tollinger and Christoph Kreutz

16 On-Line and At-Line LC-NMR and Related Micro-NMR Methods 379
Nadine Bohni, Emerson F. Queiroz and Jean-Luc Wolfender

17 New Developments of Laser Desorption Ionization Mass Spectrometry in Plant Analysis 411
Andreas Schinkovitz, Denis Seraphin and Pascal Richomme


List of Contributors xi

Preface xvii

In Memoriam
Andrew Marston xix

Part Three: Strategies for Selective Classes of Compounds 425

18 Analysis of Plant Oligo- and Polysaccharides 427
Wolfgang Blaschek

19 Analytical Strategies for Multipurpose Studies of a Plant Volatile Fraction 447
Cecilia Cagliero, Barbara Sgorbini, Chiara Cordero, Erica Liberto, Carlo Bicchi and Patrizia Rubiolo

20 Strategies for Lipid Analysis 467
Irina A. Guschina and John L. Harwood

21 HPLC Analysis of Alkaloids 485
Bras Heleno de Oliveira

22 Identification and Characterization of Hydroxycinnamates of Six Galium Species from the Rubiaceae Family 505
Rakesh Jaiswal, Marius F. Matei, Sagar Deshpande and Nikolai Kuhnert

23 Identification and Characterization of Trimeric Proanthocyanidins of Two Members of the Rhododendron Genus (Ericaceae) by Liquid Chromatography Multi-Stage Mass Spectrometry 525
Rakesh Jaiswal, Mohamed G. E. Karar and Nikolai Kuhnert

24 Strategies in the Analysis of Flavonoids 543
Celestino Santos-Buelga and Ana M. Gonzalez-Paramas

25 Coumarins – Analytical and Preparative Techniques 569
Krystyna Skalicka-Wo´zniak and Kazimierz Głowniak

26 Naphthoquinones and Anthraquinones: Chemical, Analytical, and Biological Overview 595
Nahed El-Najjar, Hala Gali-Muhtasib, Pia Vuorela, Arto Urtti and Heikki Vuorela

27 Xanthones from Marine-Derived Microorganisms: Isolation, Structure Elucidation, and Biological Activities 611
Madalena M. M. Pinto, Raquel A. P. Castanheiro and Anake Kijjoa

28 Sesquiterpenes and Other Terpenoids 633
Eirini Kouloura, Job Tchoumtchoua, Maria Halabalaki and Alexios-Leandros Skaltsounis

29 Analysis of Plant Saponins 687
Justyna Krzyzanowska, Mariusz Kowalczyk and Wieslaw Oleszek

30 Cardiotonic Glycosides 709
Liselotte Krenn

31 Plant Steroids: Occurrence, Biological Significance, and Their Analysis 727
G. M. Kamal B. Gunaherath and A. A. Leslie Gunatilaka

32 Chemical Analysis of Bryophytes 753
Yoshinori Asakawa, Agnieszka Ludwiczuk and Masao Toyota

33 Cyclotide Analysis 807
Susan E. Northfield, Aaron G. Poth, Charlotte D’Souza and David J. Craik


List of Contributors xi

Preface xvii

In Memoriam
Andrew Marston xix

Part Four: Biological Analysis 825

34 Phenotyping 827
Qiaosheng Guo and Zaibiao Zhu

35 Identification of Medicinal Plants Using DNA Barcoding 843
Xiaohui Pang and Shilin Chen

36 Transcriptome Analysis of Medicinal Plants with Next-Generation Sequencing Technologies 847
Shilin Chen and Hongmei Luo

37 Microarray 859
Chang Liu, Haimei Chen, Jianqin Li and Xiaolan Xu

38 Small RNAs 875
Shanfa Lu

39 Metabolomics 885
Jan Schripsema and Denise Dagnino

40 Proteomics and Its Research Techniques in Plants 903
Liming Yang, Haibin Xu and Shilin Chen

41 Multivariate Data Analysis 915
Bieke Dejaegher

Part Five: Drugs from Plants 947

42 In Silico-Guided Strategies for the Discovery and Rationalization of Bioactive Natural Products 949
Petra H. Pfisterer, Daniela Schuster, Judith M. Rollinger and Hermann Stuppner

43 Innovative Strategies in the Search for Bioactive Plant Constituents 967
Emerson F. Queiroz and Jean-Luc Wolfender

44 High Throughput Screening of Vegetal Natural Substances 987
Bruno David and Frederic Ausseil

45 Mycotoxins Contamination in Food: Alternative Plant Preservatives, Legislation, and Detection Methods 1011
Monica R. Zuzarte, Ana P. Martins, Maria J. Goncalves and Lígia R. Salgueiro

46 Quality Assessment of Herbal Drugs and Medicinal Plant Products 1039
Iqbal Ahmad, Mohd Sajjad Ahmad Khan and Swaranjit Singh Cameotra

Part Six: Conclusion and Perspectives 1057

47 Conclusion and Perspectives 1059

Index 1061