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Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules

Handbook of Fluorescence Spectroscopy and Imaging: From Ensemble to Single Molecules

Markus Sauer, Johan Hofkens, Jörg Enderlein

ISBN: 978-3-527-63352-4

Dec 2010

290 pages

$136.99

Description

Providing much-needed information on fluorescence spectroscopy and microscopy, this ready reference covers detection techniques, data registration, and the use of spectroscopic tools, as well as new techniques for improving the resolution of optical microscopy below the resolution gap.
Starting with the basic principles, the book goes on to treat fluorophores and labeling, single-molecule fluorescence spectroscopy and enzymatics, as well as excited state energy transfer, and super-resolution fluorescence imaging.
Examples show how each technique can help in obtaining detailed and refined information from individual molecular systems.
Basic Principles of fluorescence spectroscopy
Fluorophores and Fluorescent Labels
Fluorophore Labeling for Single-Molecule Fluorescence Spectroscopy (SMFS)
Fluorophore Selection for Single-Molecule Fluorescence Spectroscopy (SMFS) and Photobleaching Pathways
Fluorescence Correlation Spectroscopy
Excited state energy transfer
Photoinduced Electron Transfer (PET) Reactions
Super-Resolution Fluorescence Imaging
Single-molecule enzymatics
"Therefore, this book represents a valuable source of information for both specialists in single molecule
fluorescence spectroscopy and researchers new to this branch of fluorescence techniques." (Anal Bioanal Chem, 12 February 2012)