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High Performance Liquid Chromatography: Principles and Methods in Biotechnology



High Performance Liquid Chromatography: Principles and Methods in Biotechnology

Elena D. Katz (Editor)

ISBN: 978-0-471-93444-8 April 1996 536 Pages


Analytical techniques based on separation processes, such aschromatography and electrophoresis, are finding a growing range ofapplications in chemical, pharmaceutical and clinical laboratories.The Wiley Separation Science Series provides the analyst in theselaboratories with well focused books covering individualtechniques, so that they can be applied more efficiently andeffectively to contemporary analytical problems. In biotechnology,biochemistry and molecular biology, the characterization andanalysis of biomolecules such as proteins, oligosaccharides andnucleic acids are of great importance. High performance liquidchromatography (HPLC) has become one of the key analytical tools inthese fields, providing rapid purification and quantitativeanalysis of biomolecules.

High Performance Liquid Chromatography: Principles and Methods inBiotechnology covers the most important theoretical and practicalaspects of the application of HPLC to the biosciences, includingsample preparation, column selection, detection of biomolecules,preparative HPLC, and the use of HPLC for specific biomolecules.Finally, the technique is compared to alternative bioseparationmethods. The ten chapters are written by researchers active in thebiotechnology field. High Performance Liquid Chromatography:Principles and Methods in Biotechnology will be of great practicaluse to molecular biologists, biochemists and biotechnologists whouse HPLC to purify and quantify biomolecules. It will also be ofinterest to those carrying out protein and DNA characterization andanalysis.
Introduction to Chromatography in Biotechnology (C. Simpson).

The Principles of Separation by High Performance LiquidChromatography (R. Scott).

Column Selection in High Performance Liquid Chromatography (R.Eksteen).

Detection and Identification in Biochromatography (I. Krull, etal.).

Sample Preparation (A. Rizzi & I. Maurer-Fogy).

The Preparative HPLC of Biomolecules (G. Cox).

The Application of HPLC for Nucleic Acid Analysis (J. Wages &E. Katz).

High-pH Anion Exchange Chromatography of Recombinant GlycoproteinGlycans (R. Townsend).

The Application of HPLC for Proteins (K. Benedek).

Alternative Bioseparation Techniques (P. Righetti, et al.).