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Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions

Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions

Clarice M. Yentsch (Editor), Frances C. Mague (Editor), Paul K. Horan (Editor)

ISBN: 978-1-118-66944-0

Mar 2013

399 pages

Select type: O-Book

Description

Published by the American Geophysical Union as part of the Lecture Notes on Coastal and Estuarine Studies Series, Volume 25.

This volume represents a collection of pioneering papers introducing immunochemical techniques to the aquatic sciences. It is the product of a workshop entitled ""Immunochemical Approaches to Coastal, Estuarine and Oceanographic Questions"" held at the University of Southern Maine, Portland, Oct. 5-7, 1986. Funding from many sources made this workshop possible, and is gratefully acknowledged.

The reader will note great variability in both the quality and scope of the papers which we have divided into four sections:

I. Background material on the immune system (borrowed from a recent NIH publication)
II Background material related to aquatic sciences
III. Techniques
IV. Applications

LIST OF AUTHORS AND ATTENDEES

PREFACE

SPONSORS

BACKGROUND OF THE IMMUNE SYSTEM

UNDERSTANDING THE IMMUNE SYSTEM
U.S. Dept of Health and Human Services  2

BACKGROUND

WHAT DOES THE IMMUNOLOGICAL APPROACH OFFER AQUATIC RESEARCH? AN OVERVIEW
S. Pomponl, C.M. Yentsch, P.K. Horan  24

MOLECULAR BIOLOGY, SINGLE CELL ANALYSIS AND QUANTITATIVE GENETICS: NEW EVOLUTIONARY
GENETIC APPROACHES IN PHYTOPLANKTON ECOLOGY
A.M. Wood  41

TECHNIQUES

THE PRODUCTION OF MONOSPECIFIC ANTISERA TO SOFT-BOTTOM BENTHIC TAXA
E.D. Gallagher, P.A. Jumars, G.L. Taghon  74

PREPARATION AND USE OF MONOSPECIFIC ANTISERA TO CAPITELLA SP. I.
E.D. Gallagher, J.P. Grassle, D.D. Trueblood, M.G. Gleason  99

CELL AFFINITY CHROMATOGRAPHY FOR A MARINE NITRIFYING BACTERIUM
V. Heyman, B. Heyman, B.B. Ward  100

APPLICATION OF IMMUNOBLOTTING FOR DIETARY ANALYSIS
G. Zagursky, R.J. Feller  117

TWO-DIMENSIONAL ELECTROPHORESIS WITH SILVER STAINING: A SENSITIVE METHOD FOR
ANALYZING COMPLEX PROTEIN MIXTURES 130
M.F. Goldfarb  130

BREVETOXINS AND BINDING: SODIUM CHANNELS VERSUS ANTIBODIES
D.G. Baden, T.J. Mende, A.M. Szmant  134


PREPARATION OF MODEL HAPTENS TO EXPRESS COMMON EPITOPES OF PSP TOXINS
P.E. Gulre, P.H. Duquette. R.A. Amos, J.C. Behrens, M.W. Josephson, R.P. Chambers  145

MONOCLONAL ANTIBODIES (MONOABS) TO CIGUATOXIN AND RELATED POLYETHERS
Y. Hokama, S.A.A. Honda, M.N. Kobayashl, L.K. Hakagawa, L.K. Shirai, J.T. Mlyahara  155

IMMUNOFLUORESCENT REAGENTS: PREPARATION AND ANALYSIS
J.F. Daley  167

FLOW CYTOMETRY AND IMMUNOFLUORESCENCE IN AQUATIC SCIENCES
T.L. Cucci, D. Robins  184

DETECTION SCHEMES FOR ANTIGEN-ANTIBODY REACTIONS
D.J. Recktenwald, C.H. Chen, L. Chlu, M. Conrad  194

APPLICATIONS

IDENTIFICATION OF MARINE CHROOCOCCOID CYANOBACTERIA BY IMMUNOFLUORESCENCE
L. Campbell  208

AN IMMUNOFLUORESCENCE APPROACH TOWARD THE IDENTIFICATION OF ENDOSYMBIOTIC DIATOMS IN SEVERAL SPECIES OF LARGER FORAMINIFERA
J.J. Lee, Y. Chan, A. Lagzlel  230

IMMUNOFLUORESCENT CHARACTERIZATION OF THE EUKARYOTIC ULTRAPLANKTON
L. Shapiro  242

PROBES FO  242R ASSESSING SINGLE-CELL PRIMARY PRODUCTION: ANTIBODIES AGAINST RIBULOSE-1, 5-BISPHOSPHATE CARBOXYLASE (RuBPCASE) AND PERIDININ/CHLOROPHYLL A PROTEIN (PCP)
M.V. Orellana, M.J. Perry, B.A. Watson  243

EXAMINING NITRATE REDUCTION BY PHYTOPLANKTON WITH AN IMMUNOASSAY
W.M. Balch, C.M. Yentsch, B. Reguera, w. Campbell  263

A POSSIBLE IMMUNOLOGICAL APPROACH TO THE STUDY OF PLASMALEMMA REDOX ENZYMES IN PHYTOPLANKTON
G.J. Jones, F.M.M. Morel, C.M. Yentsch, E.A. Fuiikhouser  277

ANTILUCIFERASE IMMUNOGOLD LABELING OF TWO DIFFERENT ORGANELLES IN THE MARINE
DINOFLAGELLATE GONYAULAX POLYEDRA
M.-T. Nicolas, J.-M. Bassot, C.H. Johnson, J.w. Hastings  278

ENZYME-LINKED IMMUNOSORBENT ASSAY FOR METAL-BINDING PROTEINS OF UYTILUS EDULIS
G. Roesljadl, J.E. Morris  283

ENVIRONMENTAL INDUCTION OF TELEOST CYTOCHROME P-450 DETECTED BY IMMUNOCHEMICAL ANALYSIS
J.J. Stegeman  291

QUANTIFYING STOMACH CONTENTS USING IMMUNOASSAYS: A CRITIQUE
R.J. Feller, R.B. Ferguson  295

EUPHAUSIID PREDATION ON LARVAL ANCHOVY AT TWO CONTRASTING SITES OFF CALIFORNIA
DETERMINED WITH AN ELISPOT IMMUNOASSAY
G.H. Thellacker  304

ENZYME IMMUNOASSAY UTILIZING MONOCLONAL ANTIBODIES FOR IDENTIFICATION OF EUROPEAN
EEL VIRUS (EEV), AN AQUATIC BIRNAVIRUS
V. Llplpun, P. Caswell-Reno, P.W. Reno. B. Nicholson  312

MONOCLONAL ANTIBODIES TO ATLANTIC SALMON (SALMO SALAR) IMMUNOGLOBULIN: PRODUCTION
AND PRELIMINARY CHARACTERIZATION
C.E. Moody. P.W. Reno, A.E. Gagllardl  318

IMMUNOCHEMICAL EVALUATION OF THE INFECTIOUS PANCREATIC NECROSIS (IPN) CARRIER STATE
IN SALMONIDS AND OTHER SPECIES
P.W. Reno  331

REFERENCES 344

SUBJECT INDEX 389