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Industrial Bioseparations: Principles and Practice

Industrial Bioseparations: Principles and Practice

Daniel Forciniti

ISBN: 978-0-813-82085-9

Jul 2008, Wiley-Blackwell

326 pages

Select type: Hardcover

In Stock

$155.00

Description

Industrial Bioseparations offers comprehensive coverage of bioseparations including all unit operations.

This new book offers a careful balance between the fundamentals of bioseparations processing and the practical applications in industry today. It is laid out in a methodical way with preliminary chapters covering general approaches to bioseparations for commercially important biomacromolecules, thermodynamics and mass transfer principles, and following chapters addressing unit operations such as filtration and chromatography. Lab experiments are included which emphasize obtaining scale up parameters as well as commonly used operating conditions are included.

Preface.

Chapter 1. Introduction to Bioseparations.

a. Statement of the problem.

b. Engineering Developments.

c. Current approaches to bioseparations.

Chapter 2. Thermodynamics.

a. A brief introduction.

b. Thermodynamics of open systems.

c. Thermodynamic analysis of processes.

Chapter 3. Principles of Mass Transfer.

1. Diffusion.

2. Fluxes.

3. Law of conservation of mass.

4. Examples.

Chapter 4. General Properties of Bioproducts.

1. Proteins.

a. Structure.

b. Stability (solubility and aggregation).

c. Separation of complex protein mixtures by two-dimensional electrophoresis.

2. Nucleic Acids.

a. Structure.

b. Properties.

3. Laboratory experiments.

Chapter 5. Cell disruption.

1. General approach to cell disruption.

a. Types of cells.

b. Location of product.

c. Physical Chemistry Properties of Cells (as they relate to disruption).

2. Bead Mills.

3. Homogenizers.

4. Chemical Methods.

5. Biochemical Methods.

6. Laboratory experiments.

Chapter 6. Separation of Solids.

1. General Principles.

a. Flocculation of proteins.

b. Inclusion bodies.

2. Filtration.

a. Conventional Filtration.

b. Ultrafiltration.

3. Centrifugation.

4. Sedimentation.

5. Laboratory experiments.

6. Liquid/liquid extraction.

Chapter 7. Chromatography.

1. General Principles.

2. Gel permeation.

3. Ion Exchange Chromatography.

4. Hydrophobic Interactions Chromatography.

5. Affinity Chromatography.

6. Simulated Moving Bed Chromatography.

7. Laboratory Experiments.

Chapter 8. Protein Precipitation.

1. Precipitation by salting-out.

2. Isoelectric Precipitation.

3. Precipitation by non-ionic polymers.

4. Laboratory Experiments.

Chapter 9. Polishing.

1. Freeze drying.

2. Crystallization.

3. Product Formulation.

4. Laboratory Experiments.

Chapter 10. Separations in a Chip.

1. Introduction to micro-devices.

2. Fluidics.

3. Micro-reactors, micro-mixers, and valves.

4. Chromatography and electrophoresis in a chip.

5. Micro-sensors.

Chapter 11. Design of Separation cascades.

1. General Principles.

2. Examples.

3. Laboratory Projects.

Appendix. Experimental Design.

1. Introduction to Experimental Design.

2. Precision and Accuracy.

3. Descriptive Statistics.

4. Statistical Tests.

5. Examples.

Table of Contents.

Chapter 1. Introduction to Bioseparations.

a. Statement of the problem.

b. Engineering Developments.

c. Current approaches to bioseparations.

Chapter 2. Thermodynamics.

a. A brief introduction.

b. Thermodynamics of open systems.

c. Thermodynamic analysis of processes.

Chapter 3. Principles of Mass Transfer.

1. Diffusion.

2. Fluxes.

3. Law of conservation of mass.

4. Examples.

Chapter 4. General Properties of Bioproducts.

1. Proteins.

a. Structure.

b. Stability (solubility and aggregation).

c. Separation of complex protein mixtures by two-dimensional electrophoresis.

2. Nucleic Acids.

a. Structure.

b. Properties.

3. Laboratory experiments.

Chapter 5. Cell disruption.

1. General approach to cell disruption.

a. Types of cells.

b. Location of product.

c. Physical Chemistry Properties of Cells (as they relate to disruption).

2. Bead Mills.

3. Homogenizers.

4. Chemical Methods.

5. Biochemical Methods.

6. Laboratory experiments.

Chapter 6. Separation of Solids.

1. General Principles.

a. Flocculation of proteins.

b. Inclusion bodies.

2. Filtration.

a. Conventional Filtration.

b. Ultrafiltration.

3. Centrifugation.

4. Sedimentation.

5. Laboratory experiments.

6. Liquid/liquid extraction.

Chapter 7. Chromatography.

1. General Principles.

2. Gel permeation.

3. Ion Exchange Chromatography.

4. Hydrophobic Interactions Chromatography.

5. Affinity Chromatography.

6. Simulated Moving Bed Chromatography.

7. Laboratory Experiments.

Chapter 8. Protein Precipitation.

1. Precipitation by salting-out.

2. Isoelectric Precipitation.

3. Precipitation by non-ionic polymers.

4. Laboratory Experiments.

Chapter 9. Polishing.

1. Freeze drying.

2. Crystallization.

3. Product Formulation.

4. Laboratory Experiments.

Chapter 10. Separations in a Chip.

1. Introduction to micro-devices.

2. Fluidics.

3. Micro-reactors, micro-mixers, and valves.

4. Chromatography and electrophoresis in a chip.

5. Micro-sensors.

Chapter 11. Design of Separation cascades.

1. General Principles.

2. Examples.

3. Laboratory Projects.

Appendix. Experimental Design.

1. Introduction to Experimental Design.

2. Precision and Accuracy.

3. Descriptive Statistics.

4. Statistical Tests.

5. Examples.

Index

"This volume will serve as an invaluable introduction and guide to those embarking on careers in the discipline of bioseparations. Forciniti deserves our thanks for promoting knowledge of this important endeavor." (Pharmaceutical Technology, November 2, 2008)

  • comprehensive coverage of bioseparation processes including all unit operations

  • emphasizes bioseparations processes in an industrial setting and facilitates the transition from the lab into the plant

  • includes industrial case studies

  • provides mathematical descriptions for practical use in design, scaling up and optimization of the most common operations used in bioseparations.

  • describes experiments aimed at the laboratory or pilot plant determination of scaling up and operational parameters.