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Lab Exercises in Microbiology, 5th Edition

Lab Exercises in Microbiology, 5th Edition

Robert A. Pollack, Lorraine Findlay, Walter Mondschein, R. Ronald Modesto

ISBN: 978-1-119-46266-8

Jul 2018

320 pages

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The Laboratory Exercises in Microbiology, 5e by Pollack, et al. presents exercises and experiments covered in a 1 or 2-semester undergraduate microbiology laboratory course for allied health students. The labs are introduced in a clear and concise manner, while maintaining a student-friendly tone. The manual contains a variety of interactive activities and experiments that teach students the basic concepts of microbiology. The 5th edition contains new and updated labs that cover a wide array of topics, including identification of microbes, microbial biochemistry, medical microbiology, food microbiology, and environmental microbiology.

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Preface iii

Introduction: Laboratory Operations and Safety ix

Basic Rules for Microbiology Laboratory Safety xi

PART 1 General Microscopy and Aseptic Technique

1 Laboratory Safety: Introduction to the Microscope 3

2 Transfer and Isolation Techniques, Microbes in the Environment 17

PART 2 Microbial Morphology, Differential Stains

3 Cultural and Cellular Morphology 33

4 Bacterial Growth 43

5 Gram Stain and Acid-Fast Stain 53

6 Endospore Stain, Capsule Stain, and the Hanging Drop Technique 61

7 Fungi 69

8 Viruses┬ŚVisualization and Enumeration 77

9 Parasitology 85

PART 3 Microbial Control and Biochemistry

10 Microbial Sensitivity Testing 97

11 Bacterial Biochemistry 113

12 Gas Requirements of Microorganisms 123

13 Specialized Media 129

PART 4 Medical Microbiology

14 Genetics 143

15 Epidemiology 149

16 Specimen-Handling Protocols 159

17 Specific Laboratory Tests 169

18 Serology 179

PART 5 Identification of a Bacterial Unknown

19 Identification of Enteric Pathogens: Traditional Methods 191

20 Identification of Enteric Pathogens: Rapid Identification Methods 201

21 Identification of a Bacterial Unknown: The Gram-Negative Unknown 209

22 Identification of a Bacterial Unknown: The Gram-Positive Cocci 217

PART 6 Food and Environmental Microbiology

23 Identification and Quantitation of Microbial Numbers in a Water Sample 225

24 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial Numbers 231

25 Soil Microbiology 237

26 Microbial Ecology 245

27 Biofilms 251

Bibliography 261

Answer Key for the Exercise Pre-Tests 263

Appendix 1 Flow Chart for the Identification of Enterobacteriaceae 00

Appendix 2 Gram-Negative Flow Chart 00

Appendix 3 Gram-Positive Flow Chart 00

Photo Credit List 00

Index 00

Photographic Atlas 00

  • Exercises now include a brief summary that provides quick reference without re-reading the detailed text
  • Enlarged photomicrographs, cross-referenced in the text, provide clear visual guidance toward cell and microorganism morphologies and pathologies
  • Maximizes each laboratory period’s learning opportunities with well-designed, self-contained exercises
  • Includes a pre-test and quick reference/review of terminology and working definitions for each exercise
  • Features critical thinking, identification, and labeling tasks within each exercise to enhance student engagement
  • Provides fill-in work tables and charts with each Laboratory Report to allow instructors to easily view results
  • Minimizes pre-exercise reading with concise introductions using a student-friendly tone
  • Includes a Photographic Atlas of 67 full-color plates to illustrate techniques and results