DescriptionThe Laboratory Exercises in Microbiology, 4e by Pollack, et al. presents exercises and experiments covered in a 1 or 2-semester undergraduate microbiology laboratory course for allied health students. The labs are introduced in a clear and concise manner, while maintaining a student-friendly tone. The manual contains a variety of interactive activities and experiments that teach students the basic concepts of microbiology. It also covers methods that allow the safe movement or transfer of microbial cells from one type of growth environment, classification and identification of microbes, microbial biochemistry, medical, food and environmental microbiology.
Introduction: Laboratory Operations and Safety.
PART I: GENERAL MICROSCOPY AND ASEPTIC TECHNIQUE.
1 Laboratory Safety: Introduction to the Microscope.
2 Transfer and Isolation Techniques Microbes in the Environment.
PART II MICROBIAL MORPHOLOGY, DIFFERENTIAL STAINS.
3 Cultural and Cellular Morphology.
4 Bacterial Growth.
5 Gram Stain Acid-Fast Stain.
6 Endospore Stain Capsule Stain Hanging Drop Technique.
8 Viruses-Visualization and Enumeration.
PART III MICROBIAL CONTROL AND BIOCHEMISTRY.
10 Microbial Sensitivity Testing Ultraviolet Light Sensitivity.
11 Bacterial Biochemistry.
12 Gas Requirements of Microorganisms.
13 Specialized Media.
PART IV MEDICAL MICROBIOLOGY.
16 Speciman-Handling Protocols.
17 Specific Laboratory Tests.
19 Identification of Enteric Pathogens: Traditional Methods.
20 Identification of EntericPathogens: Rapid Identification Methods.
21 Identification of a Bacterial Unknown: The Gram-Negative Unknown.
22 Identification of a Bacterial.
PART V FOOD AND ENVIRONMENTAL MICROBIOLOGY.
23 Identification and Quantitation of Microbial Numbers in a Water Sample.
24 Identification of Microbes in Beef and Poultry and the Quantitation of Microbial.
25 Soil Microbiology.
26 Microbial Ecology.
Appendix 1 Flow Chart for the Identification of Enterobacteriaceae.
Appendix 2 Gram-Negative Flow Chart.
Appendix 3 Gram-Positive Flow Chart.
• New and improved color photos in the photographic atlas
• New answer key for pre-test questions
• Self-contained Lab Units. All pedagogical features will be complete for each Unit, including Lab Reports. This will help keep students focused on the topics learned in that lab.
• Variety of Questions and Activities. Includes fill-in-the blank, art labeling, coloring, identification exercises and Critical Thinking Questions - thought-provoking, open-ended questions appearing at the end of each Lab Unit that will summarize concepts learned in that lab.
• Specimen Handling Protocols lab exercise. A unique and special feature not included in any of the competing texts and is of great value to students pursuing careers in the allied health fields.
• Lab Experiments. Integrated within the discussion material, not just at the end of the chapter after students have read pages and pages of text.
• Comprehensive glossary of Working Terms and Definitions. Appears at the end of each exercise to reinforce new terms and their definitions.