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Microcharacterization of Proteins

Microcharacterization of Proteins

Roland Kellner, Friedrich Lottspeich, Helmut E. Meyer

ISBN: 978-3-527-61570-4

Jul 2008, Wiley-Blackwell

284 pages

Select type: E-Book



Both a thorough introduction and laboratory guide!

This book is based on the lectures and demonstrations presented at a successful workshop organized by the authors. Expert contributions and the support of more than a dozen companies engaged in bioanalysis and instrumentation enabled the participants to familiarize themselves with the most recent developments e.g. in protein separation and characterization (including laser desorption-ionisation mass spectrometry), fragmentation and micro sequencing.

The workshop was held in the Max Planck Institute for Biochemistry at Martinsried. It helped graduate students and researchers from academia and industry both to understand and apply themselves the most sophisticated methods available. This book offers a stimulating combination of basic concepts and practical applications - and is thus useful at all levels.

Section I: Overview

Microcharacterization of Proteins
Friedrich Lottspeich

Section II: Sample Preparation

Microseparation Techniques I: 2D-Gel-Electrophoresis
A. Görg and Reiner Westermeier
Microseparation Techniques II: Electroblotting
Christoph Eckerskorn
Microseparation Techniques III: Analysis of Peptides and Proteins by
Capillary Electrophoresis
Christine Schwer
Microseparation Techniques IV: High Performance Liquid Chromotography
Maria Serwe and Helmut E. Meyer
Chemical and Enzymatic Fragmentation of Proteins
Roland Kellner

Section III: Amino Acid Analysis

Amino Acid Analysis
Roland Kellner, Helmut E. Meyer and Friedrich Lottspeich

Section IV: Protein Sequence Analysis

The Edman Degradation
Friedrich Lottspeich, Tony Houthaeve and Roland Kellner
Analyzing Post translational Protein Modifications
Helmut E. Meyer

Section V: Bioanalytical Mass Spectrometry

Overview, Methods and Applications
W.D. Lehmann
Analysis of Biopolymers by Matrix-Assisted Laser Desorption/Ionization
(MALDI) Mass Spectrometry
Ute Bahr, Michael Karas and Franz Hillenkamp
Electrospray Mass Spectrometry
Jörg Metzger and Christoph Eckerskorn
Sequence Analysis of Proteins and Peptides by Mass Spectrometry
Christiane Weigt, Helmut E. Meyer and Roland Kellner

Section VI: Database Analysis

Protein Sequences and Sequence Databases
Hans-Werner Mewes and David G. George
Mass Spectrometrical Data for Protein Sequence Analysis
Matthias Mann
Computational Sequence Analysis
R. Apple and Denis F. Hochstrasser