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Modern Biocatalysis: Stereoselective and Environmentally Friendly Reactions

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$196.99

Modern Biocatalysis: Stereoselective and Environmentally Friendly Reactions

Wolf-Dieter Fessner (Editor), Thorleif Anthonsen (Editor)

ISBN: 978-3-527-62384-6 December 2008 400 Pages

Description

This reference covers the wide and rapidly growing field of biocatalysis. It combines complementary expertise from such areas as microbiology, enzymology, molecular biology structural biology and organic chemistry, thus highlighting the interdisciplinary nature of the subject.
With its special focus on progress and new developments towards environmentally beneficial reactions with high levels of selectivity for the production of key compound classes, this book will enlighten both chemists and biologists as to the advances and opportunities existing in enzyme catalysis.
FLUORESCENCE ASSAYS FOR BIOTRANSFORMATIONS
Introduction
Alcohol Dehydrogenases (ADHs) and Aldolases
Lipases and Esterases
Other Hydrolases
Baeyer-Villigerases
IMMOBILIZATION AS A TOOL FOR IMPROVING ENZYMES
Introduction
Adsorption/Electrostatic Interactions
Encapsulation
Covalent Binding/Cross-Linking
CONTINUOUS-FLOW MICROCHANNEL REACTORS WITH SURFACE-IMMOBILIZED BIOCATALYSTS
Introduction
Biocatalytic Synthesis Using Microreaction Technology with Free and Immobilized Enzymes
Novel Microfluidic Immobilized Enzyme Reactors
Enzymatic Hydrolysis of Lactose
Biocatalytic Process Intensification Using Microreaction Technology
ACTIVITY AND STABILITY OF PROTEASES IN HYDROPHILIC SOLVENTS
Introduction
Activity and Selectivity of Proteases in Synthesis of Carbohydrate Fatty Acid Esters
Enzyme Stability and Conformation
Solvent Engineering
IMPORTANCE OF ENZYME FORMULATION FOR THE ACTIVITY AND ENANTIOSELECTIVITY OF LIPASES IN ORGANIC SOLVENTS
Introduction
Lipase Formulations and their Activity and Enantioselectivity in Neat Organic Solvent
Why do Additives Affect the Activity and Enantioselectivity of Lipases in Organic Solvent?
DIRECT ESTERIFICATION WITH DRY MYCELIA OF MOLDS: A (STEREO)SELECTIVE, MILD AND EFFICIENT METHOD FOR OBTAINING STRUCTURALLY DIVERSE ESTERS
Mycelia and Biotransformations in Organic Media
Screening and Microbiological Aspects
Production of Acetate
Stereoselective Esterifications of Racemic Alcohols
Stereoselective Esterifications of Racemic Carboxylic Acids
Partition Phenomena and Equilibrium of Esterification Reactions
FACTORS AFFECTING ENANTIOSELECTIVITY: ALLOSTERIC EFFECTS
How to Provide Enantiopure Compounds
Factors Affecting the Enantiomeric Ratio E
Asymmetrization of Prochiral Compounds
KINETIC RESOLUTION OF SEC-ALCOHOL IN NON-CONVENTIONAL MEDIA
Introduction
SCFs -
Replacement for Organic Solvents in Biocatalysis
Effect of Pressure
Effect of the Acyl Donor/Alcohol Molar Ratio
ILs -
Solvents for Sustainable Technology in Biocatalysis
ILs, Based on the N,N'-Dialkylimidazolium Cations as Reaction Media
ILs/SCFs Biphasic Systems as Promising Media for Biocatalysis
The [bmim][PF6]/SC-CO2 System as a Reaction Media
Effect of Acyl Donor Concentration
STRATEGIES FOR THE BIOCATALYTIC LIPOPHILIZATION OF PHENOLIC ANTIOXIDANTS
Introduction
Materials and Methods
Results and Discussion
BIOCATALYSIS APPLIED TO THE SYNTHESIS OF NUCLEOSIDE ANALOGS
Introduction
Chemoenzymatic Modification of the Sugar
Resolution and Anomeric Separation
Biotransformations that Modify the Base
Transglycosylation for the Synthesis of Nucleosides
EFFICIENT FRUCTOOLIGOSACCHARIDE SYNTHESIS WITH A FRUCTOSYLTRANSFERASE FROM ASPERGILLUS ACULEATUS
Introduction
Purification of Fructosyltransferase in Pectinex Ultra SP-L
Properties of Fructosyltransferase from A. aculeatus
Immobilization of Fructosyltransferase from A. aculeatus
Fructooligosaccharide Production Using Sugar Beet Syrup and Molasses
HYDANTOIN RACEMASE: THE KEY ENZYME FOR THE PRODUCTION OF OPTICALLY PURE ALPHA-AMINO ACIDS
Introduction
Search for New Hydantoin Racemases and Molecular Characterization
Biochemical Characterization of Hydantoin Racemase Enzymes
Substrate Enantioselectivity and Kinetic Analysis of Hydantoin Racemases
Proposal for a Reaction Mechanism of Hydantoin Racemase Enzymes
Design of a Tailormade Recombinant Biocatalyst Including Hydantoin Racemase Enzymes for Optically Pure D-Amino Acid Production
CHEMO-ENZYMATIC DERACEMIZATION METHODS
Introduction
Deracemization Methods for Alpha- and Beta-Hydroxy Acids
Deracemization of Alpha-Hydroxy Nitriles
Deracemization of Alpha-Amino Acids
Useful Enzymes for Deracemization Methods
NITRILASES FROM FILAMENTOUS FUNGI
Introduction
Distribution and Evolutionary Relationship of Fungal Nitrilases
Structural Properties
Catalytic Properties
NITRILASE- AND NITRILE HYDRATASE-CATALYZED ENANTIOSELECTIVE PREPARATION OF NON-PROTEINOGENIC AMINO ACIDS
Introduction
Nitrilase Hydratase/Amidase Biotransformations
Nitrilase Biotransformations
NITRILASES IN THE ENANTIOSELECTIVE SYNTHESIS OF ALPHA-HYDROCYCARBOXYLIC ACIDS
Routes to Enantiomerically Pure Alpha-Hydroxycarboxylic Acids
Nitrilase-Mediated Hydrolysis of Cyanohydrins
A Bienzymatic Approach to Enantiopure 2-Hydrocycarboxylic Acids
Stabilization of NLases as Cross-Linked Enzyme Aggregates
Hydrocyanation and Hydrolysis in a Bienzymatic Cascade
Nitrilases Acting as Nitrile Hydratases
UF-MEMBRANE BIOREACTORS FOR KINETICS CHARACTERIZATION OF NITRILE HYDRATASE-AMIDASE-CATALYZED REACTIONS: A SHORT SURVEY
Introduction
Experiment Design
Temperature Dependence of the Nitrile Hydratase-Amidase Cascade System
CSMR Investigations
Substrate Concentration Effects on the Reaction Rate, Enzyme Stability, Substrate Conversion, and Reactor Capacity
ENZYMES CATALYZING C-C BOND FORMATION FOR THE SYNTHESIS OF MONOSACCHARIDE ANALOGS
Introduction
Recent Syntheses Involving Transketolase and Fructose-1,6-bisphosphate Aldolase
Modification of Substrate Specificity of Yeast Transketolase
NOVEL STRATEGIES IN ALDOLASE-CATALYZED SYNTHESIS OF IMINOSUGARS
Introduction
DHAP-Aldolase-Mediated Synthesis of Iminosugars from N-Cbz-Amino Aldehydes
D-Fructose-6-Phosphate Aldolase as Catalyst for Iminosugar Synthesis
BIOCATALYTIC ASYMMETRIC OXIDATIONS WITH OXYGEN
Introduction
Biocatalytic Asymmetric Oxidations with Oxidases
Biocatalytic Asymmetric Oxidations with Peroxidases
Biocatalytic Asymmetric Oxidations with Dehydrogenases
Biocatalytic Asymmetric Oxidations with Monooxygenases
Biocatalytic Asymmetric Oxidations with Dioxygenases
Biocatalytic Asymmetric Oxidations with Other Enzyms
SECOND GENERATION BAEYER-VILLIGER BIOCATALYSTS
Introduction
VBMO Enzyme Platform
Engineering of BVMOs
Baeyer-Villiger Biooxidation in Synthetic Chemistry
BVMOs in Stereoselective Sulfoxidations
Towards a Technology Platform
?With its special focus on progress and new developments towards environmentally beneficial reactions with high levels of selectivity for the production of key compound classes, this book will enlighten both chemists and biologists as to the advances and opportunities existing in enzyme catalysis.? (Organic Chemistry Portal/ Organische-Chemie, 2009)

?Books of this type are highly valuable in that each chapter is written by an acknowledged expert in the field and hence the reader is directed to the key developments and seminal contributions in the particular field.? (ChemBioChem , June 2009)