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Molecular Analysis and Genome Discovery

Molecular Analysis and Genome Discovery

Ralph Rapley (Editor), Stuart Harbron (Editor)

ISBN: 978-0-470-02019-7

Sep 2005

388 pages



This advanced level textbook provides a comprehensive overview of recent developments in the area of molecular based diagnostics (including nucleic acids, biosensors and immunoessays) of disease markers. It also covers the impact of techniques such as in vitro nucleic acid amplifications (e.g. PCR) and other amplification methods, as well as gene and biochip production and automated techniques such as fluorescent sequencing.

The book discusses key concepts where new and merging areas, including pharmacogenomics, proteomics and functional genomics, are being researched and developed. In addition, examples are given where this new area of bioscience has or may be successfully applied.


List of Contributors

1. Pharmacogenetics and Pharmacogenomics: An Overview (W. Kalow).

2. Quantitative TaqMan Real-time PCR: Diagnostic and Scientific Applications (Jörg Dötsch, Ellen Schoof and Wolfgang Rascher).

3. Hybridization Probes for Real-time PCR (Elaine Lyon).

4. An Overview of Genotyping and Single Nucleotide Polymorphisms (SNP) (Ivo Glynne Gut).

5. High-throughput Mutation Screening (Paal Skytt Andersen and Lars Allan Larsen).

6. Determination of Nucleic Acid Sequence by Pyrosequencing (Elaine Elahi and Mostafa Ronaghi).

7. An Introduction to DNA Chips (Magdalena Gabig-Ciminska and Andrzej Ciminski).

8. Overview of Microarrays in Genomic Analysis (Janette K Burgess).

9. Overview of Differential Gene Expression by High-throughput Analysis (Kin-Ying To).

10. Aptamers: Powerful Molecular Tools for Therapeutics and Diagnostics (Eva Baldrich i Rubio, Mònica Campàs i Homs and Ciara K. O'Sullivan).

11. Chip-based Proteomics Technology (Mikhail Soloviev, Richard Barry and Jon Terrett).

12. Infectomics Overview: Holistic and Integrative Studies of Infectious Diseases (Sheng-He Huang and Ambrose Jong).

13. The Drug Discovery Process (Roberto Solari).

14. Structure-based Drug Discovery (Chen-Chen Kan, Kevin Hambly and Derek A. Debe).

15. Protein Interaction-targeted Drug Discovery (Gary Hudes, Sanjay Menon and Erica A. Golemis).

16. Overview of Quantitative Structure–Activity Relationships (QSAR)(David A. Winkler).


""A compelling text that will assist a broad readership interested in keeping the pace with new developments."" (Annals of Biomedical Engineering, June 2008)

""…aims to bring together genomics and drug discovery, and to show how rapid advances in molecular analysis are bringing new tools and approaches to genome discovery."" (Genomics & Proteomics, October 2004)