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Molecular Research in Aquaculture

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Molecular Research in Aquaculture

Ken Overturf

ISBN: 978-0-813-80742-3 July 2009 Wiley-Blackwell 408 Pages

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Molecular Research in Aquaculture

Molecular research and biotechnology have long been fields of study with applications useful to aquaculture and other animal sciences. Molecular Research in Aquaculture looks to provide an understanding of molecular research and its applications to the aquaculture industry in a format that allows individuals without prior experience in this area to learn about and understand this important field.

Molecular Research in Aquaculture opens with an introductory chapter giving background information on the aquaculture industry and the development of the science and research methods to what is currently being used. From there it discusses how new, innovative techniques are now being converted and used for research in this field. Introductory chapters on basic molecular biological techniques, such as PCR, cloning, and hybridization, and their rationale provide the foundation for an in-depth look at molecular research and its specific applications. The remaining chapters review key areas of molecular research such as microarray analysis, quantitative PCR, and transgenics.

Molecular Research in Aquaculture will be a valuable reference for professionals and researchers with an interest in the development of molecular technologies and their applications to the field of aquaculture.

  • Coverage of basic molecular biological techniques and their rationale
  • In-depth look at molecular research and their applications to aquaculture
  • Valuable reference on the developments of this key area in aquaculture research

Preface vii

List of Contributors ix

Chapter 1. Convergence of Aquaculture and Molecular Biology 1
Ken Overturf

Chapter 2. Basic Molecular Laboratory Methods 15
Ken Overturf

Chapter 3. Quantitative PCR 39
Ken Overturf

Chapter 4. Aquaculture-Related Applications of DNA Microarray Technology 63
Matthew L. Rise, Zhanjiang Liu, Susan E. Douglas, Laura L. Brown, John H.E. Nash, and Margaret J. McFall-Ngai

Chapter 5. Aquaculture Genomics 103
Yniv Palti

Chapter 6. Proteomics in Aquaculture 147
Samuel A.M. Martin

Chapter 7. The Role of Model Organisms in Aquaculture Research: Transient and Permanent Advantages 175
Barrie Robison

Chapter 8. Clonal Lines and Chromosome Manipulation for Aquaculture Research and Production 195
Krista M. Nichols

Chapter 9. Issues and Methodology for Development of Transgenic Fish for Aquaculture with a Focus on Growth Enhancement 217
Robert H. Devlin, Peter A. Raven, L. Fredrik Sundström, and Mitchell Uh

Chapter 10. Molecular Regulation of Intermediary Metabolism Focusing on Utilization of Dietary Carbohydrates 261
Stéphane Panserat

Chapter 11. Muscle Regulation 279
Peggy R. Biga

Chapter 12. Microbial Genomics of Aquaculture Pathogens 315
Gregory D. Wiens

Chapter 13. Control of Reproduction 337
Gregory M. Weber

Index 383

"This book provides a basic understanding of the modern molecular techniques currently used in aquaculture research. … This book will appeal to newcomers interested in molecular techniques and provide experienced researchers with information that relates molecular function to fish physiology." (Book News, December 2009)

  • Coverage of basic molecular biological techniques and their rationale
  • In-depth look at molecular research techniques and their applications to aquaculture
  • Valuable reference on the developments of this key area in aquaculture research