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Practical HPLC Methodology and Applications



Practical HPLC Methodology and Applications

Brian A. Bidlingmeyer

ISBN: 978-0-471-57246-6 April 1993 464 Pages

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Of related interest. Trace and Ultratrace Analysis by HPLC Satinder Ahuja Written by a leading scientist in the field, this monograph provides the first definitive and technically up-to-date treatment of the theory, equipment, and applications of chemistry's most powerful reliable analytical technique. Coverage includes an encyclopedic compendium of common substances that require trace and ultratrace analysis, and features clear discussion of such important topics as considerations for HPLC equipment, sensitive detectors, sample preparation, method development, selectivity and computer-based optimizations, optimizing detectability, and much more. 1991 (0 471-51419-5) 432 pp. High Performance Liquid Chromatography in Biotechnology Edited by William S. Hancock Analytical chemists, biochemists, and chemical engineers will find this up-to-date guide to HPLC's recent developments essential for enhancing on-the-job technical expertise. Extensive coverage includes the broad applications of HPLC, ranging from major chromatographic techniques (including reversed phase, ion exchange, affinity and hydrophobic interaction chromatography) to specific separations such as those in monoclonal antibody and nucleic acid purification. Techniques for quality control programs and advanced technology are also discussed. 1990 (0 471-82584-0) 564 pp. Unified Separation Science J. Calvin Giddings This advanced text/monograph brings together for the first time the variety of techniques used for chemical separations by outlining their common underlying mechanisms. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form, facilitating analysis of alternative separation techniques and the factors integral to separation power. The first six chapters provide background material applicable to a wide range of separation methods, while the final five chapters illustrate specific techniques and methods. 1991 (0 471-52089-6) 320 pp.
Liquid Chromatography as a Research Tool.

The Chromatogram and What Contributes to It.

Approaching the Problem.

Developing the Separation.

Considerations for Proper Operation of a LiquidChromatograph.

Gradient Elution Chromatography.

Experiment 1: Demonstrating the Fundamentals.

Experiment 2: Normal-Phase Chromatography.

Experiment 3: Effect of Column Length and Recycle.

Experiment 4: Gel Permeation Chromatography Using Dual Detectors(UV and RI).

Experiment 5: Developing a Reverse Phase ChromatographicSeparation.

Experiment 6: Quantitation.

Experiment 7: Monitoring Kinetics.

Experiment 8: Preparative Liquid Chromatography.

Experiment 9: Analysis of Essential Oils (Steam Distillates).

Experiment 10: Gradient Elution.