Skip to main content

Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells

Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells

Silvia Maria Doglia (Editor), Marina Lotti (Editor)

ISBN: 978-1-118-85503-4

Apr 2014

288 pages

$107.99

Description

Focuses on the aggregation of recombinant proteins in bacterial cells in the form of inclusion bodies—and on their use in biotechnological and medical applications

The first book devoted specifically to the topic of aggregation in bacteria, Protein Aggregation in Bacteria: Functional and Structural Properties of Inclusion Bodies in Bacterial Cells provides a large overview of protein folding and aggregation, including cell biology and methodological aspects. It summarizes, for the first time in one book, ideas and technical approaches that pave the way for a direct use of inclusion bodies in biotechnological and medical applications.

Protein Aggregation in Bacteria covers:

  • Molecular and cellular mechanisms of protein folding, aggregation, and disaggregation in bacteria
  • Physiological importance and consequences of aggregation for the bacterial cell
  • Factors inherent to the protein sequence responsible for aggregation and evolutionary mechanisms to keep proteins soluble
  • Structural properties of proteins expressed as soluble aggregates and as inclusion bodies within bacterial cells both from a methodological point of view and with regard to their similarity with amyloids
  • Control of the structural and functional properties of aggregated proteins and use thereof in biotechnology and medicine

Protein Aggregation in Bacteria is ideal for researchers in protein science, biochemistry, bioengineering, biophysics, microbiology, medicine, and biotechnology, particularly if they are related with the production of recombinant proteins and pharmaceutical science.

Contributors vii

Preface xi

Introduction to the Wiley Series in Protein and Peptide Science xiii

1 Fundamentals of Protein Folding 1
Vladimir N. Uversky

2 Recruiting Unfolding Chaperones to Solubilize Misfolded Recombinant Proteins 63
Rayees U.H. Mattoo and Pierre Goloubinoff

3 Osmolytes as Chemical Chaperones to Use in Protein Biotechnology 77
Ario de Marco

4 Inclusion Bodies in the Study of Amyloid Aggregation 93
Anna Villar-Piqué and Salvador Ventura

5 Protein Aggregation in Unicellular Eukaryotes 117
Marina Caldara, Joris Winderickx, and Vanessa Franssens

6 Structural Properties of Bacterial Inclusion Bodies 151
Antonino Natalello, Diletta Ami, and Silvia Maria Doglia

7 Residue-Specific Structural Studies of Inclusion Bodies 181
Christian Wasmer, Marielle Wälti, Yongli Chen, and Lei Wang

8 Biomedical Applications of Bacterial Inclusion Bodies 203
Imma Ratera, Spela Peternel, Joaquin Seras-Franzoso, Olivia Cano-Garrido, Elena García-Fruitós, Rafael Cubarsí, Esther Vazquez, José Luis Corchero, Escarlata Rodríguez-Carmona, Jaume Veciana, and Antonio Villaverde

9 Aggregation of Recombinant Proteins: Understanding Basic Issues to Overcome Production Bottlenecks 221
Marina Lotti and Loredano Pollegioni

10 Fusion to a Pull-Down Module: Designing Enzymes to Form Biocatalytically Active Insoluble Aggregates 247
Bernd Nidetzky

Index 263