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Protein Engineering Handbook



Protein Engineering Handbook

Stefan Lutz (Editor), Uwe Theo Bornscheuer (Editor)

ISBN: 978-3-527-66701-7 July 2012 1015 Pages


Unparalleled in size and scope, this new major reference integrates academic and industrial knowledge into a single resource, allowing for a unique overview of the entire field. Adopting a systematic and practice-oriented approach, and including a wide range of technical and methodological information, this highly accessible handbook is an invaluable 'toolbox' for any bioengineer. In two massive volumes, it covers the full spectrum of current concepts, methods and application areas.

Guidelines for the Functional Analysis of Engineered and Mutant Enzymes
Engineering Enantioselectivity in Enzyme-Catalyzed Reactions
Mechanism and Catalytic Promiscuity: Emerging Mechanistic Principles for Identification and Manipulation of Catalytically Promiscuous Enzymes
Phi-Value Analysis of Protein Folding Transition States
Protein Folding and Solubility: Pathways and High-Throughput Assays
Protein Dynamics and the Evolution of Novel Protein Function
Gaining Insight into Enzyme Function through Correlation with Protein Motions
Structural Frameworks Suitable for Engineering
Microbes and Enzymes: Recent Trends and New Directions to Expand Protein Space
Inteins in Protein Engineering
From Prospecting to Product -
Industrial Metagenomics Is Coming of Age
Computational Protein Design
Assessing and Exploiting the Persistence of Substrate Ambiguity in Modern Protein Catalysts
Designing Programmable Protein Switches
The Cyclization of Peptides and Proteins with Inteins


A Method for Rapid Directed Evolution
Evolution of Enantioselective Bacillus subtilis Lipase
Circular Permutation of Proteins
Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): A Versatile Tool for Generating Targeted Libraries
Protein Engineering by Structure-Guided SCHEMA Recombination
Chimeragenesis in Protein Engineering
Protein Generation Using a Reconstituted System
Equipping in vivo Selection Systems with Tunable Stringency
Protein Engineering by Phage Display
Screening Methodologies for Glycosidic Bond Formation
Yeast Surface Display in Protein Engineering and Analysis
In Vitro Compartmentalization (IVC) and Other High-Throughput Screens of Enzyme Libraries
Colorimetric and Fluorescence-Based Screening
Confocal and Conventional Fluorescence-Based High Throughput Screening in Protein Engineering
30 Alteration of Substrate Specificity and Stereoselectivity of Lipases and Esterases
Altering Enzyme Substrate and Cofactor Specificity via Protein Engineering
Protein Engineering of Modular Polyketide Synthases
Cyanophycin Synthetases
Biosynthetic Pathway Engineering Strategies
Natural Polyester-Related Proteins: Structure, Function, Evolution and Engineering
Bioengineering of Sequence-Repetitive Polypeptides: Synthetic Routes to Protein-Based Materials of Novel Structure and Function
Silk Proteins -
Biomaterials and Bioengineering
“Stefan Lutz and Uwe Bornscheuer did a great job! By convincing many of the key players in the field to contribute to this two volume masterpiece, they managed to assemble basically all aspects of modern protein engineering technology under one roof, that is, between four hard covers.” (Angewandte Chemie, February 2010)

“The Protein Engineering Handbook provides the first comprehensive overview of the highly cross-disciplinary field of protein engineering that many researchers and students have been eagerly awaiting.” (ChemBioChem, August 2009)