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Proteins in Solution and at Interfaces: Methods and Applications in Biotechnology and Materials Science



Proteins in Solution and at Interfaces: Methods and Applications in Biotechnology and Materials Science

Juan M. Ruso (Editor), Ángel Piñeiro (Editor)

ISBN: 978-1-118-52317-9 January 2013 500 Pages



Explores new applications emerging from our latest understanding of proteins in solution and at interfaces

Proteins in solution and at interfaces increasingly serve as the starting point for exciting new applications, from biomimetic materials to nanoparticle patterning. This book surveys the state of the science in the field, offering investigators a current understanding of the characteristics of proteins in solution and at interfaces as well as the techniques used to study these characteristics. Moreover, the authors explore many of the new and emerging applications that have resulted from the most recent studies. Topics include protein and protein aggregate structure; computational and experimental techniques to study protein structure, aggregation, and adsorption; proteins in non-standard conditions; and applications in biotechnology.

Proteins in Solution and at Interfaces is divided into two parts:

  • Part One introduces concepts as well as theoretical and experimental techniques that are used to study protein systems, including X-ray crystallography, nuclear magnetic resonance, small angle scattering, and spectroscopic methods
  • Part Two examines current and emerging applications, including nanomaterials, natural fibrous proteins, and biomolecular thermodynamics

The book's twenty-three chapters have been contributed by leading experts in the field. These contributions are based on a thorough review of the latest peer-reviewed findings as well as the authors' own research experience. Chapters begin with a discussion of core concepts and then gradually build in complexity, concluding with a forecast of future developments.

Readers will not only gain a current understanding of proteins in solution and at interfaces, but also will discover how theoretical and technical developments in the field can be translated into new applications in material design, genetic engineering, personalized medicine, drug delivery, biosensors, and biotechnology.




1 X-Ray Crystallography of Biological Macromolecules: Fundamentals and Applications 3
Antonio L. Llamas-Saiz and Mark J. van Raaij

2 Nuclear Magnetic Resonance Methods for Studying Soluble, Fibrous, and Membrane-Embedded Proteins 23
Victoria A. Higman

3 Small-Angle X-Ray Scattering Applied to Proteins in Solution 49
Leandro Ramos Souza Barbosa, Francesco Spinozzi, Paolo Mariani, and Rosangela Itri

4 Analyzing the Solution State of Protein Structure, Interactions, and Ligands by Spectroscopic Methods 73
Veronica I. Dodero and Paula V. Messina

5 Resolving Membrane-Bound Protein Orientation and Conformation by Neutron Reflectivity 99
Hirsh Nanda

6 Investigating Protein Interactions at Solid Surfaces—In Situ, Nonlabeling Techniques 113
Olof Svensson, Javier Sotres, and Alejandro Barrantes

7 Calorimetric Methods to Characterize the Forces Driving Macromolecular Association and Folding Processes 139
Conceic¸ ˜ao A.S.A. Minetti, Peter L. Privalov, and David P. Remeta

8 Virtual Ligand Screening Against Comparative Models of Proteins 179
Hao Fan

9 Atomistic and Coarse-Grained Molecular Dynamics Simulations of Membrane Proteins 193
Thomas J. Piggot, Peter J. Bond, and Syma Khalid


10 Preparation of Nanomaterials Based on Peptides and Proteins 209
Yujing Sun and Zhuang Li

11 Natural Fibrous Proteins: Structural Analysis, Assembly, and Applications 219
Mark J. van Raaij and Anna Mitraki

12 Amyloid-Like Fibrils: Origin, Structure, Properties, and Potential Technological Applications 233
Pablo Taboada, Silvia Barbosa, Josue Juarez, Manuel-Alatorre Meda, and Výctor Mosquera

13 Proteins and Peptides in Biomimetic Polymeric Membranes 283
Alfredo Gonzalez-Perez

14 Study of Proteins and Peptides at Interfaces By Molecular Dynamics Simulation Techniques 291
David Poger and Alan E. Mark

15 A Single-Molecule Approach to Explore the Role of the Solvent Environment in Protein Folding 315
Katarzyna Tych and Lorna Dougan

16 Enhanced Functionality of Peroxidases By Its Immobilization at The Solid–Liquid Interface of Mesoporous Materials and Nanoparticles 335
Jose Campos-Teran, Iker Inarritu, Jorge Aburto, and Eduardo Torres

17 Superactivity of Enzymes in Supramolecular Hydrogels 353
Ye Zhang and Bing Xu

18 Surfactant Proteins and Natural Biofoams 365
Malcolm W. Kennedy and Alan Cooper

19 Promiscuous Enzymes 379
Luis F. Olguin

20 Thermodynamics and Kinetics of Mixed Protein/Surfactant Adsorption Layers at Liquid Interfaces 389
Reinhard Miller, E.V. Aksenenko, V.S. Alahverdjieva, V.B. Fainerman, C.S. Kotsmar, J. Kragel, M.E. Leser, J. Maldonado-Valderrama, V. Pradines, C. Stefaniu, A. Stocco, and R. Wustneck

21 Application of Force Spectroscopy Methods to the Study of Biomaterials 429
Chuan Xu and Erika F. Merschrod S.

22 Protein Gel Rheology 437
Katie Weigandt and Danilo Pozzo

23 Exploring Biomolecular Thermodynamics in Aqueous and Nonaqueous Environments using Time-Resolved Photothermal Methods 449
Randy W. Larsen, Carissa M. Vetromile, William A. Maza, Khoa Pham, Jaroslava Miksovska