Skip to main content

RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals

RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C. elegans, Drosophila, and Mammals

Ute Schepers

ISBN: 978-3-527-60437-1

Mar 2006

336 pages

$181.99

Description

This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids.
In easy-to-follow, step-by-step protocols you will learn
* how RNAi works in worms, flies and mammals,
* how to design the most efficient RNAi constructs,
* how to achieve transient, stable and conditional RNAi in cell cultures,
* how to determine the efficiency of an RNAi experiment,
* and how to use RNAi for gene therapy.
All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.
Introduction
- Mechanism of RNAi
- RNAi as a Tool for Functional Genomics
RNAi in C.elegans
- Practical Application
- Target Evaluation
- Synthesis and Delivery of dsRNA
- Genome-wide Screening
RNAi in Drosophila
- Practical Application
- Synthesis and Delivery of dsRNA
- RNAi in Cell Lines
RNAi in mammals
- Transient RNAi in Cell Culture
- Design and Synthesis of siRNAs
- Delivery of siRNAs into Cells
- Analysis and Validation of siRNAs
- RNAi with Hairpin RNAs
- Inducible RNAi
- RNAi in mice
- Screening Methods
RNAi Resources
- Web Resources
- List of Suppliers
- Glossary of Terms
"I would recommend this book to anyone wishing to establish RNAi-mediated gene silencing in their lab." Comparative & Functional Genomics