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The Model Legume Medicago truncatula




The Model Legume Medicago truncatula

Frans J. de Bruijn

ISBN: 978-1-119-40916-8 January 2020 Wiley-Blackwell 1264 Pages

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Fully covers the biology, biochemistry, genetics, and genomics of Medicago truncatula

Model plant species are valuable not only because they lead to discoveries in basic biology, but also because they provide resources that facilitate translational biology to improve crops of economic importance. Plant scientists are drawn to models because of their ease of manipulation, simple genome organization, rapid life cycles, and the availability of multiple genetic and genomic tools. This reference provides comprehensive coverage of the Model Legume Medicago truncatula. It features review chapters as well as research chapters describing experiments carried out by the authors with clear materials and methods. Most of the chapters utilize advanced molecular techniques and biochemical analyses to approach a variety of aspects of the Model.

The Model Legume Medicago truncatula starts with an examination of M. truncatula plant development; biosynthesis of natural products; stress and M. truncatula; and the M. truncatula-Sinorhizobium meliloti symbiosis. Symbiosis of Medicago truncatula with arbuscular mycorrhiza comes next, followed by chapters on the common symbiotic signaling pathway (CSSP or SYM) and infection events in the Rhizobium-legume symbiosis. Other sections look at hormones and the rhizobial and mycorrhizal symbioses; autoregulation of nodule numbers (AON) in M. truncatula; Medicago truncatula databases and computer programs; and more.

  • Contains reviews, original research chapters, and methods
  • Covers most aspects of the M. truncatula Model System, including basic biology, biochemistry, genetics, and genomics of this system
  • Offers molecular techniques and advanced biochemical analyses for approaching a variety of aspects of the Model Legume Medicago truncatula
  • Includes introductions by the editor to each section, presenting the summary of selected chapters in the section
  • Features an extensive index, to facilitate the search for key terms

The Model Legume Medicago truncatula is an excellent book for researchers and upper level graduate students in microbial ecology, environmental microbiology, plant genetics and biochemistry. It will also benefit legume biologists, plant molecular biologists, agrobiologists, plant breeders, bioinformaticians, and evolutionary biologists.

Section 1.

1.1. General Introduction

Section 2. Overview Chapters

2.1. A Snapshot of Functional Genetic Studies in Medicago truncatula
Jerome Verdier

2.2. Medicago truncatula as an ecological, evolutionary, and forage legume model: New directions forward
Eric von Wettberg

Section 3. M. truncatula plant development

3.1. Seed Development: Introduction

3.1.1. A physiological perspective of late maturation processes and establishment of seed quality in Medicago truncatula seeds
Julia Buitink and Jerome Verdier

3.1.2. Medicago truncatula an informative model to investigate the DNA damage response during seed germination
Alma Balestrazzi

3.1.3. Transcriptional networks in early Medicago truncatula embryo development
Ray Rose

3.1.4. Embryo development and the oil and protein bodies in Medicago truncatula
Ray Rose

3.1.5. Role of thioredoxins and NADP-thioredoxin reductases in legume seeds and seedlings
Francoise Montrichard

3.1.6. Seed shape quantification in the model legumes: Methods and applications
Emilio Cervantes

3.1.7. The underlying processes governing seed size plasticity: Impact of endoploidy on seed coat development and cell expansion in Medicago truncatula
Sergio Ochatt with Mona Darmency

3.2. Root development Introduction

3.2.1. Nitrate signaling pathway via the transporter MPF6.8 involves abscisic acid for the regulation of primary root elongation in Medicago truncatula
Anis Limami

3.2.2. SCARECROW and SHORT6ROOT show an overlapping expression pattern in the Medicago truncatula nodule central meristem
Henk Franssen

3.2.3. Lateral root formation and patterning in Medicago truncatula
Sandra Bensmihen

3.2.4. Modulation of root elongation by Abscisic acid and lateral root organ defective/numerous infections and polyphenolics modulate root elongation via reactive oxygen species in Medicago truncatula
Jeanne Harris

3.2.5. FYVE and PH protein domains present in MtZR1, a PRAF protein, modulate the development of roots and symbiotic root nodules of Medicago truncatula via potential phospholipids signaling
Eric Boncompagni

3.3. Leaf development Introduction

3.3.1. Compound leaf development in Medicago truncatula
Rujin Chen

3.3.2. Mechanistic insights into STENOFOLIA-mediated leafblade outgrowth in Medicago truncatula.
Million Tadege

3.4. Flower development Introduction

3.4.1. Genetic control of flowering time in legumes
James Weller and McNight

3.4.2. Forward and reverse screens to identify genes that control vernalization and flowering time in Medicago
Joanna Putterill

3.4.3. MtNAM regulates floral organ identity and lateral organ separation in Medicago truncatula

Section 4. Biosynthesis of natural products

Section 4 Introduction

4.1. Organization and regulation of triterpene saponin biosynthesis in Medicago truncatula
Alain Goossens

4.2. Saponins in Medicago truncatula: structures and activities
Pedro Da Silva

4.3. Saponin synthesis in Medicago truncatula plants: CYP450-mediated formation of sapogenins in the different plant organs
Carla Scotti

Section 5. Stress and M. truncatula

5.1. Introduction

5.1.1. Genetic variation of transgenerational plasticity of offspring germination in response to salinity stress and the seed transcriptome of Medicago truncatula
Maren Friesen

5.1.2. Isolation and functional characterization of salt- induced RC12-like genes from Medicago sativa and Medicago truncatula
Oingchuan Yang

5.1.3. Rhizobial symbiosis influences response to early salt and drought stress of the Medicago truncatula root proteome
Stefanie Wienkoop

5.1.4. Deciphering the role of the alternative respiration under salt stress in Medicago truncatula
M. Ribas-Carbo

5.1.5. Unraveling the effect of arsenic on the model Medicago-Ensifer interaction: a transcriptomic meta-analysis
Eloisa Pajuelo

5.1.6. Dual oxidative stress control involving antioxidant defence system and alternative oxidase pathways within the model legume Medicago truncatula under biotic and abiotic constraints
Haythem Mhadhbi

5.2. Biotic Stress: Interaction of M. truncatula with pathogens and pests

5.2.1. Introduction Medicago truncatula and other annual Medicago spp.- interactions with root and foliar, oomycete and viral pathogens
M. Barbetti Deciphering resistance mechanisms to the root rot disease of legumes caused by Aphamyces euteiches with Medicago truncatula genetic and genomic resources
Christophe Jacquet and Maxime Bonhomme Medicago truncatula as a model organism to study conserved and contrasting aspects of symbiotic and pathogenic signaling pathways
Sebastian Schornachand Aleksandr Gavrin Tools and strategies for the genetic and molecular dissection of Medicago truncatula against Fusarium wilt disease
Louise Thatcher, Brendan Kidd and Karam Singh Medicago truncatula as a model host for genetic and molecular dissection of resistance to Rhizoctonia solani
Jonathan Anderson, Brendan Kidd, Karam Singh Phosphorus control of plant interactions with mutualistic and pathogenic microorganisms: a mini-review and a case study of the Medicago truncatula B9 mutant
HoaiNam Truong The Medicago truncatula-Ralstonia solanacearum pathosystem opens up many research perspectives
Fabienne Vailleau

5.2.2. Aphid Stress Introduction Medicago-Aphid interactions
Karam Singh, Lars Kamphuis plus Glen Powell Medicago truncatula-pea aphid interaction in the context of global climate change
Yuchen Sun

5.2.3. Interactions with other pathogens and parasites Characterization of defense mechanisms to parasitic plants in the model Medicago truncatula
Diego Rubiales and Maria Angeles Medicago truncatula host/ nonhost rust interactions
Maria Vaz Patto with Dr. Rubiales Medicago truncatula as a model to study powdery mildew resistance
Diego Rubiales, Nicolas Rispail Antifungal defensins from Medicago truncatula: structure-activity relationships, modes of action and biotech applications
Dillip Shah Leaf me alone: Medicago truncatula defenses against foliar lepidopteran herbivores
Jacqueline Bede

Section 6. The M.truncatula-Sinorhizobium meliloti symbiosis

6.1. Introduction of symbiotic nitrogen fixation

6.2. Signalling and early infection events in the Rhizobium-legume symbiosis

6.2.1. The role of the flavonoid pathway in Medicago truncatula in root nodule formation A Review
Ulrike Mathesius

6.2.2. Expression and function of the Medicago truncatula lysine motif receptor-kinases (LysM-RLK) gene family in the legume-rhizobia symbiosis
Julie Cullimore

6.2.3. Nod factor hydrolysis in Medicago truncatula: Signal inactivation or formation of secondary signals?
Christian Staehelin

6.2.4. The Medicago truncatula E3 Ubiquitin Ligase PUB1 negatively regulates rhizobial and arbuscular mycorrhizal symbioses through its ubiquitination activity
Christine Herve

6.2.5. Encoding nuclear calcium oscillations in root symbioses
Myriam Charpentier

Section 7. Symbiosis of Medicago truncatula with arbuscular mycorrhiza

7.1. Signalling and infection events in the arbuscular mycorrhiza-M.truncatula symbiosis

7.1.1. The symbiosis of Medicago truncatula with arbuscular mycorrhizal fung
Didier Reinhardt

7.1.2. Phytohormone signaling in arbuscular mycorhiza development
Caroline Gutjahr

7.1.3. Laser Micro dissection of Arbuscular Mycorrhiza
Erik Limpens

7.1.4. Overlapping expression patterns and differential transcript levels of phosphate transporter genes in arbuscular mycorrhizal, Pi-fertilized and phytohormone-treated Medicago truncatula roots
Philipp Franken

Section 8. The common symbiotic signalling pathway (CSSP or SYM).

8.1. The Common Symbiotic Signalling Pathway
Frederic Debellé

8.2. Contribution of model legumes to knowledge of actinorhizal symbiosis
Didier Bogusz and Claudine Franche

8.3. Della proteins are common components of the symbiotic rhizobial and mycorrhizal signaling pathways
Ertao Wang

Section 9. Infection events in the Rhizobium-legume symbiosis.

9.1. Genes induced during the rhizobial infection process. Introduction

9.1.1. Comparative analysis of the tubulin cytoskeleton rearrangements in nodules of Medicago truncatula and Pisum sativum
Viktor Tsyganov

9.1.2. Post-transcriptional reprogramming during root nodule symbiosis
Marie Eugenia Zanetti

9.1.3. Knotted-Like Homeobox 3: A new regulator of symbiotic nodule development
Mary Osipova

9.1.4. Features of Sinorhizobium meliloti exopolysaccharide succinoglycan required for successful invasion of Medicago truncatula nodules
Kathryn Jones

9.1.5. Infection thread development in model legumes
Daniel Gage

9.2. Rhizobial release, symbiosomes and bacteroid formation

9.2.1. The Defective in Nitrogen Fixation genes of Medicago truncatula reveal key features in the intracellular association with rhizobia
Dong Wang

9.2.2. Terminal bacteroid differentiation in the Medicago-Rhizobium interaction- a tug of war between plant and bacteria
Andreas Haag and Peter Mergaert

9.2.3. More than antimicrobial: Nodule Cysteine-rich peptides maintain a working balance between legume plant hosts and rhizobia bacteria during nitrogen-fixing symbiosis
Huairong Pan

9.2.4. Functional dissection of Medicago truncatula nodules with activated defence 1 in maintenance of rhizobial endosymbiosis
Zongming Zhang

9.2.5. Which role for Medicago truncatula non-specific lipid transfer proteins in rhizobial infection?
Tiziana Pandolfini

9.2.6. Syntaxin MTSYP132 defines symbiotic membranes in Medicago truncatula root nodules
Janine Sherrier

9.3. Nodule and Bacteroid functioning

9.3.1. Metal transport in Medicago rhizobia-infected cells
Manuel Gonzalez-Guerrero

9.3.2. Inhibition of glutamine synthetase leads to a fast transcriptional activation of defence responses in root nodules
Helena Carvalho

9.3.3. N-feedback regulation is synchronized with nodule carbon alteration in Medicago truncatula under excessive nitrate or low phosphorus conditions
Lam-Son Phan Tran

9.4. Bacteroid senescence

9.4.1. Involvement of proteases during nodule senescence in leguminous plants
Eric Boncompagni

9.4.2. Senescence of Medicago truncatula root nodules: NO balance
Eliane Meilhoc

9.4.3. Medicago truncatula ESN1, a key regulator of nodule senescence and symbiotic nitrogen fixation.
Rujin Chen

9.5. Structure of indeterminate M. truncatula nodules

9.5.1. Development and structures of the meristems of roots and indeterminate nodules. Introduction The root apical meristem in M. truncatula
Barbara Lotocka The meristem in indeterminate root nodules of Faboideae
Barbara Lotocka

Section 10. Hormones and the rhizobial and mycorrhizal symbioses

10. Section 10 intro

10.1 Phytohormone regulation of Medicago- Rhizobia interactions. A review
Ulrike Mathesius

10.2. Plant hormones play common and divergent roles in nodulation and arbuscular mycorrhizal symbioses
Eloise Foo

10.3. Auxins and other phytohormones as signals in arbuscular mycorrhiza formation
Jutta Ludwig-Muller

10.­4. Ethylene-responsive miRNAs in roots of Medicago truncatula identified by high-throughput sequencing at the whole genome level
Mingui Zhao

10.5. Hormone-induced nodule-like structures in land plants: An update
Arjit Mukherjee

10.6. Structural studies of Medicago truncatula proteins participating in cytokinin signal transduction and nodulation
Milosz Ruszkowski

10.7. Identification and network enabled characterization of auxin response factor genes in Medicago truncatula
Rajeev Azad

Section 11. Autoregulation of nodule numbers (AON) in M. truncatula

11.1. The autoregulation gene SUNN mediates changes in nodule and lateral root formation in response to nitrogen through changes of shoot-to-root auxin transport
Ulrike Mathesius

Section 12. Genetics and Genomics of M. truncatula

12.1. Genetic map of M. truncatula

12.2. The genome sequence of M. truncatula

12.2.1. An improved genome release (Version Mt4.0) for the model legume Medicago truncatula
Chris Town

12.2.2. The sequenced genomes of Medicago
Nevin Young

12.3. Quantitive Trait Loci mapping

12.3.1. QTL analysis of seed germination and seedling pre-emergence growth under abiotic stresses
Beatrice Teulat-Merah

12.3.2. Unravelling the determinants of freezing tolerance in Medicago truncatula: a first step towards improving the response of crop legumes to freezing stress using translational genomics
Bruno Delbrell

12.4. Genome-wide association and Medicago truncatula GWAS and Medicago truncatula

12.4.1. Multi-locus GWAS and genome-wide composite interval mapping (GCIM)
Yuan-Ming Zhang

12.4.2. Genome Wide Association Mapping and Medicago truncatula
Maxime Bonhomme

12.4.3. Validating genome-wide association candidates controlling quantitative variation in nodulation
Shaun Curtin and Nevin Young

12.5. Transposons, gene instability and gene tagging

12.5.1. ClassII transposable elements in Medicago truncatula
Dariusz Crzebelus

12.6. M. truncatula and evolution

12.6.1. Comparative genomics suggests that an ancestral polyploidy event leads to enhanced root nodule symbiosis in the Papilionoideae
Yuan-Ming Zhang

12.6.2. Patterns of polymorphism, recombination and selection in Medicago truncatula
Peter Tiffin Timothy Paape

12.6.3. Genome-wide determination of poly(A) sites in Medicago truncatula: evolutionary conservation of alternative poly’A) site choice
A.G. Hunt QingshunLi

12.7. The M. truncatula genome and translational genomics

12.7.1 GBS based genome-wide association and genomic selection for alfalfa (Medicago sativa) forage quality improvement
Paolo annicchiarico

12.8. Genomic and genetic markers in M. truncatula

12.8.1. Development and characterization of Simple Sequence Repeat (SSR) markers based on RNA-sequencing of Medicago truncatula and in silico mapping onto the M. truncatula genome
Zan Wang

12.8.2. Genome-wide development of microRNA-based SSR markers in Medicago truncatula with their transferability analysis and utilization in related legume species
Wenxian Liu

12.9. Small RNA’s in M. truncatula

12.9.1. Small RNA diversity and roles in model legumes
Martin Crespi

12.9.2. Small RNA deep sequencing identifies novel and salt-stress-regulated microRNAs from roots of Medicago sativa and Medicago truncatula
Qingchuan Yang

12.9.3. MiR171h restricts root symbioses and shows like its target NSP2 a complex transcriptional regulation in Medicago truncatula
Emanuel Devers

12.9.4. MicroRNA-Based biotechnology for plant improvement
Baohong Zhang

12.9.5. Computational investigation of small RNAs in the establishment of root nodules and arbuscular mycorrhiza in leguminous plants
Ming Chen

12.10. Mutagenesis, forward and reverse genetics in M. truncatula

12.10.1. Isolation and characterization of non-transposon symbiotic nitrogen fixation mutants of Medicago truncatula
Peter kalo with Rujin Chen

12.10.2. Targeted mutagenesis by an optimized Agrobacterium-delivered CRISP/Cas9 system in the model legume Medicago truncatula
Lifang Niu

12.10.3. Whole genome sequencing of symbiotic nitrogen fixation mutants from the Medicago truncatula Tnt1 mutant population to identify relevant Tnt1 and MERE1 insertion sites
Rebecca Dickstein

12.10.4. A simple method for genetic crossing in Medicago truncatula
Alexandre Boscari

12.10.5. An artificial-micro RNA system based on an endogenous microRNA of Medicago truncatula to unravel the function of root endosymbiosis related gene
Emanuel Devers

12.11. Transcriptomics in M. truncatula

12.11.1. Synergism and symbiosis: Unpacking complex species interactions using transcriptomic approaches
Michelle Afkhami

12.11.2. Comparative genomic and transcriptomic analyses of legume genes controlling the nodulation process
Marc Libault

12.11.3. Transcriptional profiling of genes and pathways associated with osmotic and salt stress responses in Medicago truncatula
Wen-Hao Zhang

12.12. Medicago truncatula proteomics

12.12.1. Organelle protein changes in arbuscular mycorrhizal Medicago roots as deciphered by subcellular proteomics.
Ghislaine Recorbet

12.12.2. Leveraging proteome and phosphoproteome to unravel the molecular mechanisms of legume-rhizobia symbiosis
Jean Michel Ané

12.12.3. Application of Bottom-up and Topdown proteomics in Medicago spp.
Jenny Renaut

12.12.4. Medicago truncatula: Local response of the root nodule proteome to drought stress
Esther M. Gonzalez

12.12.5. Comparative proteomic analysis reveals differential root proteins in Medicago sativa and Medicago truncatula in response to salt stress
Qinchuan Yang

12.13. M. truncatula metabolomics

12.13.1. Multifaceted investigation of metabolites during nitrogen fixation in Medicago via high resolution MALDI-MS imaging and ESI-MS
Lingjun Li

Section 13. Medicago truncatula databases and computer Programs

13.1. MTGD: The Medicago truncatula genome Database
Vivek Krishnakumar

13.2. Transcriptional factor databases for legume plants
Lam Son Phan Tran

13.3. Plant Omics Data Center: An integrated web repository for interspecies gene expression network with NLP based curation
Kentaro Yano

Section 14. M. truncatula and transformation

14.1. Recent advances in Medicago spp. genetic engineering strategies
Massimo Confalonieri

14.2. Agrobacterium-mediated transformation of Medicago truncatula cell suspensions
Anelia Iantcheva

14.3. Ray Rose New Title: The Jemalong 2HA line used for Medicago truncatula transformation: hormonology and epigenetics
Ray Rose

14.4. Composite Medicago truncatula plants harbouring Agrobacterium rhizogenes-transformed roots reveal normal mycorrhization by Glomus intraradices
Bettina Hause