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The Science of Laboratory Diagnosis, 2nd Edition

The Science of Laboratory Diagnosis, 2nd Edition

John Crocker (Editor), David Burnett (Editor)

ISBN: 978-0-470-85913-1

Dec 2005

564 pages



This fully revised and updated edition of The Science of Laboratory Diagnosis provides a concise description of all common laboratory tests available in medical practice with notes on their application, the accuracy of each test, the historical background to the adoption of various tests and their effectiveness in diagnosis.
  • Well illustrated, with clear headings, tables, flow charts and pathology slides, most in full colour
  • Provides an accessible reference book in which relevant information can be found easily
  • Page design facilitates rapid assimilation of principles and key facts
  • All the chapters have been updated and new material has been introduced to cover recently developed techniques, such as fluid-based cytology, telepathology and proteomics

The Science of Laboratory Diagnosis, Second Edition is an essential primary reference source for everyone working in a clinical laboratory. This book is essential reading for pathologists, biomedical scientists, medical laboratory scientific officers and all clinicians involved in laboratory research.

Reviews of the First Edition:

"The text is concise, wide-ranging and easy to digest. The ease of extraction of the important facts make it an ideal source of information for use in a variety of situations from the postgraduate examination to the clinical directors' board meeting." BULLETIN OF THE ROYAL COLLEGE OF PATHOLOGISTS

"The editors have done a marvellous job, more than fulfilling their stated aim of producing a volume describing the multidisciplinary state of modern pathology which will be of interest to a wide range of readers. … I was particularly impressed by the many tables and flow charts, which can be used as aids to decision making." JOURNAL OF CLINICAL PATHOLOGY

"This is an excellent book to dip into and get a feel for techniques used in the other disciplines of pathology." ANNALS OF CLINICAL BIOCHEMISTRY

Preface to the First Edition.

Preface to the Second Edition.

Contributors Lists.

Section 1: Histopathology.

1: Specimen Handling and Preparation for Routine Diagnostic Histopathology (Paul J. Smith and Janine L. Warfield).

2: General stains (Barrie Sims).

3: Enzyme Histochemistry (Trevor Gray and Neil Hand).

4: Immunohistochemistry (Jane Starczynski and John Crocker).

5: Electron Microscopy in Pathology (Brian Eyden).

6: Light Microscopy (Brian Boullier).

7: Quantitative Methods in Tissue Pathology (Peter W. Hamilton and Derek C. Allen).

8: Proliferation Markers in Histopathology (Cheryl E. Gillett and Diana M. Barnes).

Section 2: Cytology.

9: Cytopathology (Adrian T. Warfield).

Section 3: Microbiology – Bacteriology

10: Microscopy in Bacteriology: Application and Sample Preparation (Dugald R. Baird).

11: Culture Media in Bacteriology (Eric Y. Bridson).

12: Identification of Bacteria (Andrew Hay ).

13: Automated tests in Bacteriology (Paul C. Boreland).

14: Bacteriology Molecular Techniques: sample preparation and application (Richard E. Holliman and Julie D. Johnson).

15: Other tests in bacteriology (Darrel Ho-Yen).

16: Control of antimicrobial chemotherapy (Ian M. Gould).

Section 4: Microbiology – Virology,Mycology and Parasitology.

17: Microscopy in virology: application and sample preparation (Marie M. Ogilvie).

18: Electron microscopy in virology (Hazel Appleton).

19: Tissue culture (William L. Irving and Alan Pawley).

20: Serological tests in virology (Goura Kudesia).

21: Automated tests in virology (Roger P. Eglin).

22: Molecular techniques in virology (Paul E. Klapper).

23: Other tests in Virology (Alex W. L. Joss).

24: Mycology (David W. Warnock).

25: Parasitology (Robert W. A. Girdwood).

Section 5: Haematology.

26: Blood Cell and Bone Marrow Morphology (Supatrik Basu).

27 The Principles of Automated Blood Cell Counters (Graham Martin).

28 Methods for the Identification of the Haemoglobinopathies (Nick Jackson and Anne Sermon).

29: Special von Willebrand Factor investigations (Mohammad S. Enayat).

30 Platelet Investigations (Steven Walton and Peter Rose).

31 A Hospital Transfusion Service (Steven Walton and Peter E. Rose).

32 Flow Cytometry and Molecular Biology in Haematology (Ian Chant).

33: Haematinic Investigations (Frank Wells).

34: Laboratory Investigations of Haemolysis (Paul Revell).

35: Laboratory investigation of haemostasis (Peter E. Rose and Catherine Caveen).

Section 6: Cytogenetics..

36: Chromosome Banding and Analysis (Mervyn Humphreys).

37: Fluorescence in situ hybridization (Ivor Hickey).

Section 7: Clinical Chemistry.

38: The acquisition, uses and interpretation of clinical biochemical data (William J. Marshall).

39: Immunoassay in clinical biochemistry (Joan Butler and Susan M. Chambers).

40: Ion selective electrodes (Alan D. Hirst).

41: Light absorption, scatter and luminescent techniques in routine clinical biochemistry (Bernard F. Rocks).

42: Analytical atomic spectrometry (Andrew Taylor).

43: Dry Reagent Chemistry Techniques (Jessica A. Schroeder, Jacqueline C. Osypiw and Gordon S. Challand).

44: Separation techniques (Roy Sherwood).

Section 8: Immunology.

45: Protein Assays (David Burnett).

46: Complement Assays (Jonathan North and Keith Whaley).

47: Cellular Immunology (Aarnoud Huissoon)

48: Immune Complex Diseases and Cryoglobulins (Siraj A. Misbah).

49: HLA typing (Mark Hathaway).

Section 9: Molecular Pathology.

50: Laser Capture Microdissection (Orla Sheils, Paul Smyth, Esther O’Regan, Stephen Finn, Richard Flavin and John O’Leary).

51: TaqMan PCR Gene Analysis (Cara M. Martin, Orla Sheils and John J. O’Leary).

52: In-cell PRC (John J. O’Leary, Cara M. Martin and Orla Sheils).

53: High-density SNP and cDNA array analysis (Orla Sheils, Cara M. Martin, Paul Smyth, Jon Sherlock, Stephen Finn, Esther O’Regan, and John O’Leary).

54: CGH Array Analysis of Human Tissues (Esther O’Regan, Paul Smyth, Stephen Finn, Cara M. Martin, Orla Sheils and John J. O’Leary),

55 DNA Sequencing (Cara M. Martin, Steve Picton and John O’Leary).


"…effective use of color enhances the text0-which by itself is excellent…a valuable addition to any laboratory medicine library." (Doody's Health Services)

"...the book will be of interest to a wide variety of readers, is beautifully produced with colour photographs and line diagrams which clearly explain the practicalities described in the invaluable reference book...well worth the money..." (The British Toxicology Society, No 27, Winter 2005)

  • Well illustrated, with clear headings, tables, flowcharts and pathology slides, in full colour
  • Provides an accessible reference book in which relevant information can be found easily
  • Effective page design helps make understanding of principles and key facts easier