What's Wrong With My Mouse?: Behavioral Phenotyping of Transgenic and Knockout Mice, 2nd Edition
DescriptionDr. Jacqueline N. Crawley, author of the First and Second Editions of What’s Wrong with My Mouse? Behavioral Phenotyping of Transgenic and Knockout Mice,continues to field calls and e-mails from molecular geneticists who ask: how do I run behavioral assays to find out what’s wrong with my mouse?
Turn to What’s Wrong with My Mouse? to discover the wealth of mouse behavioral tasks and to get the guidance you need to select the best methods and necessary controls. Chapters are organized by behavioral domain, including measurements of general health, motor functions, sensory abilities, learning and memory, feeding and drinking, reproductive, social, emotional, and reward behaviors in mutant mice. Throughout the chapters, new behavioral tasks and new research discoveries have been added, bringing the Second Edition up to date with the latest science. In addition, the Second Edition includes two new chapters:
"Neurodevelopment and Neurodegeneration" discusses mouse behavioral tasks relevant to neurodevelopmental diseases, such as mental retardation and autism, and to neurodegenerative diseases, such as Alzheimers, Parkinsons, Huntingtons, and amyotrophic lateral sclerosis.
"Putting It All Together" recommends strategies for optimizing a battery of behavioral phenotyping tests to address your specific hypotheses about gene functions.
The final chapter, "The Next Generation," examines new and emerging technologies.
Throughout the book, the use of behavioral testing equipment is illustrated with photographs, diagrams, and representative data. Examples of behavioral tasks successfully applied to transgenic and knockout mouse models are provided, as well as references to the primary literature and step-by-step methods protocols. These features, along with a comprehensive index, listings of database and vendor websites, and an extensive list of references, make this book a valuable and practical resource for students and researchers.
Preface to the First Edition xi
Chapter 1 Designer Mice 1
Scope and sourcebooks
Chapter 2 Of Unicorns and Chimeras 10
How to generate a line of transgenic or knockout mouse for behavioral phenotyping
Chapter 3 General Health 42
Give your mouse a physical
Chapter 4 Motor Functions 62
Open field, holeboard, rotarod, balance, grip, circadian activity, circling, stereotypy, ataxic gait, seizures
Chapter 5 Sensory Abilities 86
Olfaction, vision, hearing, taste, touch, nociception
Chapter 6 Learning and Memory 110
Morris swim task, spatial mazes, cued and contextual conditioning, conditioned taste aversion, conditioned eyeblink, olfactory discrimination, social recognition, passive avoidance, schedule controlled operant tasks, motor learning, attention
Chapter 7 Feeding and Drinking 164
Daily consumption, restricted access, choice tests, microstructural analysis
Chapter 8 Reproductive Behaviors 186
Sexual and parental behaviors
Chapter 9 Social Behaviors 206
Social interaction, nesting, grooming, juvenile play, aggression
Chapter 10 Emotional Behaviors: Animal Models of Psychiatric Diseases 226
Mouse models of fear, anxiety, depression, schizophrenia
Chapter 11 Reward 266
Self-administration of addictive drugs, conditioned place preference
Chapter 12 Neurodevelopment and Neurodegeneration 290
Assaying behaviors in infant, juvenile, and aged mice
Chapter 13 Putting It All Together 322
Choice of tests, order of testing, number of mice, equipment, housing and testing environment
Chapter 14 The Next Generation 344
Conditional and inducible mutations, viral vector gene delivery, RNA silencing, quantitative trait loci analysis, DNA microarrays, chemical mutagenesis, gene therapy, ethical issues
"The book successfully brings together the field of behavioral neuroscience and the rewards and insights that can be gained using this approach. I would recommend that every behavioral scientist has at least two copies, one for their own use and one that will be on permanent load to their students, post-doctoral students and colleagues in molecular biology." (Trends in Pharmaceutical Sciences)
"the overall impression is of a helpful guide taking the reader step by step through the complexities of animal behavioral research." (Nature Neuroscience)
"succeeds as a useful introduction to the realm of behavioral phenotyping for those interested in creating or using the large and increasing number of promising targeted mutant mice." (Trends in Neurosciences)
- Answers the questions: What are the best tests? What controls should be used? How do you avoid potential hidden pitfalls, false positives and negatives, and lurking artifacts? Which statistical tests can be applied?
- Includes a new chapter on Developmental and Neurodegenerative Diseases, covering mental retardation and inborn errors of metabolism through to Alzheimer's disease and stroke
- Provides brand new coverage of practical recommendations for choosing tasks to evaluate a new mutant line (Putting It All Together), including complete guidelines for high-throughput screening
- Discusses implications of the completion of the mouse genome sequence, microarray technology, new gene delivery methods, stem cell transplantation, proteomics, and high throughput approaches in chemical mutagenesis studies
- Expands discussion to the latest studies in learning and memory, autism and social phobias, olfaction, drug addiction, withdrawal, and spinal cord injury
- Includes numerous new figures showing new behavioral tests, equipment, and data
- Each chapter concludes with extensive, up-to-date references and suggestions for background reading