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Working in Biosafety Level 3 and 4 Laboratories: A Practical Introduction



Working in Biosafety Level 3 and 4 Laboratories: A Practical Introduction

Manfred Weidmann (Editor), Nigel Silmann (Editor), Patrick Butaye (Editor), Mandy Elschner (Editor)

ISBN: 978-3-527-33467-4 December 2013 Wiley-Blackwell 152 Pages


The first training manual for new staff working in BSL3/4 labs.

This guide is based on a course developed in 2007 by the EU COST action group 28b which serves as a standard for many courses BSL3/4 training courses worldwide. The four-day course consists of lectures and practical training with the lecturers covering all the different possibilities of organising a BSL-3/4 lab including the adaptation to the local requirements of biosafety, safety at work, and social regulations.

This book covers bio-containment, hazard criteria and categorisation of microbes, technical specifications of BSL-3 laboratories and ABSL-3 laboratories, personal protective gear, shipping BSL-3 and BSL-4 organisms according to UN and IATA regulations, efficacy of inactivation procedures, fumigation, learning from a history of lab accidents, handling samples that arrive for diagnostic testing and bridging the gap between the requirements of bio-containment and diagnostics. Course participants can not only use the book for their actual training event but it will remain a useful reference throughout their career in BSL3/4 labs.
Introduction (Manfred Weidmann)
Laboratory Biosafety in Containment Laboratories (Annette A Kraus and Ali Mirazimi)
Hazard criteria and categegoisation of microbes: Classification systems (Nigel J. Silman)
Technical and practical aspects of BSL-3 laboratories (Frank T. Hufert and Manfred Weidmann)
Animal biosafety level 3 facility - enhancements when dealing with large animals (Abad F.X., Solanes D., Domingo M.)
Personal Protective Equipment (Nigel J Silman)
Shipping of infectious substances according IATA DGR regulations (Mandy Elschner and Martin Heller)
Disinfection and decontamination (Patrick Butaye)
Fumigation of Spaces (Nigel J. Silman)
Learning from a history of laboratory accidents (Manfred Weidmann)
Bridging the gap between requirements of bio-containment and diagnostics (Manfred Weidmann, Frank T.Hufert, Nigel Silman)
Risk Assessment Procedures (Asa Szekely Björndal)
Biosecurity (Juergen Mertsching)