In this incisive, concise overview of this booming field, the editors -- two of the leading figures in the field with a proven track record -- combine their expertise to provide an invaluable reference on the topic.
Following a treatment of transcriptome analysis, the book goes on to discuss replacement and mutation analysis, gene silencing and computational analysis. The whole is rounded off with a look at emerging technologies.
Each chapter is accompanied by a concise overview, helping readers to quickly identify topics of interest, while important, carefully selected words and concepts are explained in a handy glossary.
Equally accessible to both experienced scientists and newcomers to the field.
Table of contents
Single-Cell Expression Profiling
AFLP-based RNA Fingerprinting
The SAGE family: SAGE, LongSAGE and SuperSAGE
DeepCAGE: High-Throughput Gene Expression Analysis for Transcription Start Site and Promoter Identification
Paired End diTagging (GIS-PET) for Transcriptome Characterization
High-Throughput Functional Screening of Genes in planta
Microarrays as Tools to Decipher Transcriptomes in Symbiotic Interactions
BeadArray-based Gene Expression Analysis
Genome-wide Analysis of mRNA expression by Fluorescent Differential Display
Quantitative Real-time RT-PCR
GENE SILENCING, MUTATION ANALYSIS, AND FUNCTIONAL GENOMICS
RNA Interference for Functional Genomics
Extending Functional Genomics: VIGS for Model and Crop Plants
Identification of Expressed Genes by Mutation: TILLING
Identification of Expressed Genes by Mutation: Transposon Tagging
Fast Neutron Mutagenesis
Bioinformatics Tools to Discover Co-expressed Genes in Plants
Integration and Phylogenomics
Structural, Functional, and Comparative Annotation of Plant Genomes
Large-Scale Genomic Sequence Comparisons and Gene Identification with ClustDB
Nanotechnologies and Fluorescent Proteins for Functional Genomics
Next Generation Sequencing Technologies
454 Sequencing for Functional Genomics
"... I recommend this book to any plant researcher who is interested in incorporating functional genomics approaches or who is looking to expand their repertoire. I especially liked that most chapters include detailed 'recipes', which show that anyone with a standard wet laboratory can do most of the procedures... a very useful volume..." -The Quarterly Review of Biology, March 2010